The Study Of Rumen Microflora And The Gene Expression Differences In Lamb

In this study, there were each six lambs of Gansu alpine fine-wool sheep and itsF1hybrid, research on the genetic background Determination of the effect of rumenfermentation parameters, using PCR-DGGE method for detecting a rumen microbialflora, the use of real-time PCR analysis techniques the host and ruminal fatty acidtransport, muscle development and fat deposition expression of related genes.Obtained the following results:1.By measuring the Gansu Alpine Merino (group A) and South Gan F1generation lambs (group B) of rumen microbial VFAs, the results showed that: therewas no significant difference between group A and group B sheep in addition tobutyric acid(P>0.05), but there are some differences between the rest of them. Amongthem, the Acetic acid of group A was significantly higher than group B (P<0.01); thePropionate of group B was significantly higher than group A (P<0.01); the total acidof group A was significantly higher than group B (P<0.05); the acetic acid/propionateof A group was significantly higher than group B (P<0.01). By measuring GansuAlpine Merino (group A) and South Gan F1generation lambs (group B) of rumenmicroorganisms of various nitrogen content, the results showed that: there was nosignificant difference between group A and group B of protein nitrogen(P>0.05), ureanitrogen, ammonia nitrogen and total nitrogen of group B were significantly higherthan those of group A (P<0.01).2.Technical analysis using PCR-DGGE with two groups sheep of rumenmicrobial flora diversity, the results show that group A and B sheep of PCR-DGGEpatterns were no similarity; Gansu Alpine Merino and Camarines F1rumen microbialdiversity indices were3.61and3.53, and the difference was not significant (P>0.05).Sequence analysis showed that the21DGGE fingerprint advantage16S rDNAsequence were greater than97%similarity with the strip32in the known genesequence in GenBank sequence.3.Using RT-PCR technique to detect the difference of rumen microbial flora inthe two groups, the results showed that: Sheep PPARγ gene expression levels in GansuAlpine Merino adipose tissue was significantly higher than South Gan F1generationlambs(P<0.05); sheep MCT1and MCT4in the rumen wall tissue gene expressionlevel was significantly higher than South Gan F1(P<0.05), whereas there was nodifferences of MCT2genes (P>0.05); There was no difference of the expression ofsheep IGF-Ⅰ Rgenes in longissimus muscle tissue(P>0.05), but the expression ofGansu alpine Merino in Camarines higher than the F1lambs, and the expression ofIGF-Ⅰ gene of Gansu alpine Merino was significantly higher in Camarines F1generation lambs muscle(P<0.05); the expression of IGF-Ⅰ gene was no differencesbetween the two groups in sheep liver(P>0.05).Completion of the work is to understand the different genetic background ofrumen microbial flora and host gene expression variation, creating a different geneticbackground of early weaning and feeding sheep technology to provide data support.