The Soil Microflorain And Microbial Functional Diversity In Greenhouses Of Different Planting Years

Solar-supported greenhouse cultivation is important in cold area vegetableproduction in China. Due to high utilization intensity and limited suitable crops,rotation in greenhouse is actually impossible, which results in soil degradation,productivity decrease, serious pests and disease problems as well as notable change insoil microflorain and soil physical and chemical properties. We determined thenutrient contents and microorganism quantity of soil from greenhouses having beenplanted for one, five, ten and fifteen years in Gaolan County in middle GansuProvince. The relationships between microbial function diversity, microorganismquantity and soil nutrients were analyzed, with the purpose of providing theoreticalbasis for reasonable fertilization and sustainable management of the greenhouseproduction in the region. The main results are as follows:(1)In the greenhouse soil of different planting years,3species microorganismscompositions are the same. The most is bacteria, then is actinomyces, fungi is at least.With the increasing of planting year, the microorganisms are increasing first and thendecreasing, like a invert saddle and in the10th years get the peak. OM, total nitrogenand total phosphorus of the soil in the10th years reach the max. The pH of thegreenhouse soil is lower than naked soil. In different planting years, solar greenhousesoil’s OM and total nitrogen have significantly or heavily significantly positivecorrelation with bacteria and actinomyces and total microorganisms. Total potassiumhas heavily significantly positive correlation with fungi, total phosphorus hassignificantly positive correlation with actinomyces. It is visible that soilmicroorganisms’quality and activity have directly influence to soil ecosystem and soilfertility.(2)In the greenhouse soil of different planting years about soil functional bacteria,with the increasing of planting year, denitrifying bacteria are decreasing first and thenincreasing, nitrite bacteria and cellulose decomposition bacteria are increasing firstand then decreasing. Phosphorus bacteria are sustaining increasing and significantly higher than control group after planting15years. Ammonifying bacteria’s version is“high-low-high”. It is visible that ammonifying bacteria occupies absolute advantage,then phosphorus bacteria, denitrifying bacteria’s quality is the least.(3)Giving pesticide to different planting year-greenhouse soil, soilmicroorganisms’ quality has a larger version. The quality of greenhouse soil fungiafter abamectin dealing with, its version is “low-high-low”. After planting10and15years, the quality of soil’s fungi and actinomycetes are all higher than the controlgroup. The quality of greenhouse soil actinomycetes after cypermethrin dealing with,its version is sustaining increasing, fungi’s quality is inhibited significantly and thenincreasing. The quality of greenhouse soil fungi after chlorothalonil dealing with, itsversion is inhibited, actinomycetes are increasing with the planting years increasing.(4)Using Biolog ECO slab method to analyze soil microflora’s functional diversitywith different planting years, the result shows as follows. Soil microorganisms takingadvantange of different origin of carbon’s ability is decreasing with the planting yearsincreasing. Soil microorganisms’ ACWD with6kinds of origin of carbon shows,origin of carbon’s using is gaining with the training time increasing. The greenhousesoil microorganisms’ ability of taking advantage of carbohydrate and amines oforigins of carbon that planting10years and15years is all higher than control group.The greenhouse soil microorganisms’ ability of taking advantage of amino acids oforigins of carbon that planting1year and10years and15years is all higher thancontrol group.