Leaf Color Performance And MYB Transcription Factor Gene Cloning Of Acer Truncatum ‘Luhong No.1’

Acer truncatum is deciduous trees of the Acer(Aceraceae). It is an important autumn leaves tree species in North China, and widely used in garden greening. At present, people study the Acer truncatum is mainly in the cultivation and utilization, in the research of mechanism of color development was less, the cloning and expression of genes involved in coloration of leaf in Acer palmatum are not reported up to now. This greatly limits the development of molecular breeding and leaf color gene engineering of Acer truncatum.Because anthocyanin play a key role informing colorful leaf in color leaf plant, in this study,using the research group breeding of the Acer truncatum ‘Luhong No.1’as materials, and common maple varieties as control, determined the content of anthocyanin during the same period, leaf color parameters of Each of the Department and mother, With the related of the development of red transcription factor gene cloning, the main results are as follows:1. In the leaf color phase, Acer truncatum ‘Luhong No.1’and ordinary control anthocyanin showed first increased and then decreased,but the‘Luhong No.1’anthocyanin content obvious than normal control of Acer truncatum anthocyanin content high. The relationship between anthocyanin content and leaf color L, a, b analysis, found that the content of anthocyanin depends on how much the leaf color parameter a value.2. After five consecutive years of observation, found that the vast majority of individuals‘Luhong No.1’open pollinated progeny families autumn leaves become red or orange red. It is obviously different from other families. a value analysis of Leaf color parameters shows that there is no big difference between annual that trait with genetic stability, the leaf color gene regulation, and the degree of dominance higher3. Kit method, TRIZOL reagent and modified CTAB were chosen for comparison analysis of leaves total RNA extraction from ‘Luhong No.1’, to obtain high yield, high quality leaves total RNA, These results demonstrate that the modified CTAB method is more suitable for the total RNA isolation from the leaf of ‘Luhong No.1’.4. By using the method of RT-PCR and RACE-PCR, success of the MYB transcription factor gene that regulate and control anthocyanin expressing, total length of this gene was831 bp, encoding 276 amino acids; the protein molecular weight is 32169.9Da, its molecular formula is C1430H14052N2247O406S14, atomic number 4510, isoelectric point was 9.44; the protein is hydrophobic, no signal peptide. BLAST comparison In the NCBI, found that therewas high homology with other species of the nucleotide sequence of the cloned and deduced amino acid sequence, which suggested the sequence is MYB gene and named AthMYB.GenBank accession number 1825712.