Effects Of Temperature On Storage Proteins Of Bradysia Odoriphaga And Of Soil Water Content On The Survival And Reproduction Of The Pest

The paper dealt with on the storage protein of Bradysia odoriphaga, which focus on the primary features such as molecular weight, subunit molecular weight, dynamic variations and the different environment effects on the storage protein content of B. odoriphaga larva and pupa. At the same time, studies were done on the influence of soil water content on the B.odoriphaga. The mainly results were as follows:1. The molecular weight measurement and dynamic variations of B. odoriphaga storage proteinsBy collecting the hemolymph protein samples of B. odoriphaga during different stages of larval and pupa, and then through SDS-PAGE and native-PAGE, the studies showed the storage protein of B. odoriphaga belonged to hexamerins and the molecular weights were471.2 k D. It has only one single type of subunit, and the subunit molecular weight is 77.9 k D.The contents of storage proteins in hemolymph increased during the later forth instar stage and then continued decreasing till to stage of the late pupa. Through the comparison between the male and female, the contents of female storage protein are obvious high than the male in the stage of pre-pupa, and its contents similar to each other in the stage of early pupa.2. The dynamic variations of storage proteins in the hemolymph and fatbody under different temperaturesBy collecting the hemolymph and fat body protein samples of B. odoriphaga under different temperature treatments, and then carrying on SDS-PAGE, the studies found that the samples in 25 ℃ treatment had more storage proteins in B. odoriphaga hemolymph compared with which under 20 ℃ treatments. With the 4 different temperature treatment(15℃, 20℃, 25℃ and 30℃), the storage proteins in the hemolymph of B. odoriphaga increased with the increase of temperature at the end of the larva stage. It also increased with the temperature increase at the pre-pupa stage. There was no obvious difference among the different treatments at the later pupa stage. There are a few storage proteins during the fourth stage of larva and a little difference in the content of storage proteins in fat body among the different temperature treatment in the stage of pre-pupa and the later pupa. The temperature has a little effect on the contents of storage proteins in fat body at different stages. Thecontents of storage proteins in fat body increased when it decreased in the hemolymph.3. Effects of soil water content on the survival and the fecundity of B. odoriphagaThe research sets a series of different soil water contents to observe the changing of survival rate and reproduction of B. odoriphaga. It turns out the best soil water content for B.odoriphaga’s living ranges from 15% to 22%. The different of soil water content has great influence on the development and reproduction of B. odoriphaga, and the effects vary among the different developmental stages. The optimal soil water content for the egg stage was10%~22%, and the best for the larval stage was 15%~20%. The suitbale soil water content for the pupa ranges from 5% to 20%. The largest average production of eggs at 20% soil water content was 128.