Transformation Of Physcomitrella Patens LEA Protein Gene In Maize Hi-Ⅱ And Analysis Of Detection

Maize is an important feed crop and it is widely distributed around the world. Drought, cold, high salinity and other abiotic stresses are all limiting factors affecting fed corn production. Late embryogenesis abundant proteins (LEA proteins) are a series of proteins which accumulate in the late embryonic development and LEA proteins are considered to have the function of improving plant resistance. In this experiment, the exogenous gene is LEA8981 gene which is isolated from Physcomitrella patens, and we use the constitutive Actin promoter to express LEA8981 in maize Hi-Ⅱ.After getting the positive transgenic plants, we do the drought treatment between the transgenic plants and the wild type to verify their drought resistance. Use gene gan to bombard maize callus and the fragments with LEA8981 expression cassette and Bar gene expression cassette are transformed into maize. We get a positive transgenic line, and analyze its drought resistance. The result shows that LEA protein transgenic line has more drought resistance than the wild type. And after drought treatment transgenic plants have higher proline content and lower MDA content than the wild type. Use Agrobacterium EHAagll to infect maize immature embryos and transform the LEA8981 and Bar gene into maize. Eventually, we get 10 positive transgenic lines. RT-PCR and Southern Blot prove that LEA gene has successfully inserted into maize genome and expressed stably, In this study, transgenic plants are successfully obtained LEA8981 drought resistance function are verified in maize and the transformation process is optimized, which provides a new way of thinking to LEA protein resistance research and provides a new direction for the directional improvement fed corn.