The Study On The Distribution Of AMF Species And Soil Factors In Mining Subsidence Area Of Bulianta Coal Mine

Arbuscular mycorrhizal fungi (AMF) have a wide range of infectivity on plants so that more than 80% of terrestrial plants can form AM. We intend to study the distribution of AMF species and explore the change law of AMF and soil factors with the increasing of mining subsidence period, seasons changing, and the cracks of the earth’s surface appeared by taking the mining area of Bulianta coal mine as the research sample and AMF and soil factors as the research objects. The work of this paper will provide theoretical foundation for choosing high quality AMF species to carry out the mining area land reclamation and vegetation restoration. The research results of this paper were concluded as follows:1. In mining subsidence area of Bulianta coal mine, totally 20 species of nine families and 18 genera of plants were isolated, the proportion was 80%. Among them, 1 genus and 1 species of Asclepiadaceae,3 genera and 3 species of Cheropodiaceae were not infected by AMF.2.1n mining subsidence area of Bulianta coal mine, totally 13 species of 5 genera AMF were isolated. The species of AMF in the surface-crack area was less than that in the non surface-crack area. The cracking of soil surface reduced the AMF spore density and species richness.3. The proportion of the three kinds of soil microbial quantity from high to low was bacteria, actinomycetes and fungi. The soil microbial quantity in the surface-crack area was less than that in the non surface-crack area.4. Minging of subsidence and cracking of the surface influnced soil phosphatase activity and soil physical and chemical properties.