| The purpose of this experiment was to study the injury of Aflatoxin B1(AFB1)on liver and immune functions of chicken and the alleviation effect of Astragalus polysaccharides(APS) on that injury. Then provided a theoretical basis for the clinical application of APS on the alleviation of mycotoxin injury for poultry.In order to investigate the effect of different concentrations of AFB1 on liver and immune functions in chicken, 64 1-day-old HY-line Brown female chickens were randomly assigned to 4 dietary treatments, then continuously added 0mg/kg, 2.8 mg/kg, 4.2 mg/kg, 5.6 mg/kg AFB1 for 7 days in each group respectively. Results showed that 2.8 mg/kg AFB1 for 7 days can significantly decrease the immune organ index. Liver appeared enlargement and bleeding, liver index increased significantly. The structural integrity of liver tissue and vessel wall was damaged, And the injury aggravated along with the increase of time and dose.To test the prevention of APS on liver and immune functions injury induced by AFB1 in chicken, 120 one-day-old chickens were randomly divided into five groups, group I was the control group, group II was the positive control group with AFB1, and group III, IV, V were the different dose of APS(0.1, 0.5, 1 g/L). AFB1 of 2.8 mg/kg was added to the diet for 7 days in Group II-V, simultaneously receiving APS of 0.1, 0.5 and 1g/L in drinking water respectively for 21 days in group III-V. The immune organs were weighed and the blood samples were collected on days 7, 14, 21 and 28. The results indicated that the three doses of APS all have a significant protection on liver and immune functions injury induced by AFB1. The middle dose of APS group at 7-day-old chicken, the thymus and spleen index and TP, ALB, IL-1, IL-2, SOD, EPO contents and GSH-Px activity in serum was significantly picked up(P<0.05 or P<0.01), but the liver index, ALT, AST, ALP activity and TBIL, MDA contents were significantly fell back to normal(P<0.01). The level of IL-3 in serum at the age of 14 days, The index of bursa of Fabricius at the age of 21 days and the PLT content in blood at the age of 28 days rose significantly(P<0.05 or P<0.01). The high dose group of APS group has the same effect as the middle one, except the PLT content at the age of 7 days had a apparent increase(P<0.01).To study the therapeutic effect of APS on liver and immune functions injury induced by AFB1 in chicken, 90 one-day-old chickens were randomly divided into five groups, group I was the control group, group II was the positive control group with AFB1, and groups III, IV, V were the different doses of APS(0.1, 0.5, 1 g/L). AFB1 of 2.8 mg/kg was added to the diet for 7 days in Group II-V, after that receiving APS of 0.1, 0.5 and 1g/L in drinking water respectively for 21 days in group III-V. The immune organs were weighed and the blood samples were collected on days 14, 21, 28. Results indicated that the high dose group of APS had good therapeutic effect on liver and immune functions injury induced by AFB1 in chicken. At the age of 14 days, compared with AFB1 positive group, high dose of APS group can significantly picked up the thymus index, liver index, IL-1, SOD and GSH-Px activity(P<0.05 or P<0.01), and biochemical indexes(ALT, AST, ALP, TBIL, TP, ALB) were restored to normal, the content of MDA was significantly decreased(P<0.01) and the index of spleen and bursa of Fabricius, immune factors(IL-2, IL-3, EPO) also rose significantly at the age of 21 days(P<0.05 or P<0.01). While the spleen index, IL-3 and WBC about the middle dose of APS group started to pick up at the age of 28 days. The low dose of APS group of chicken at the age of 14 days, all indexes had no significant difference compared with the positive control group(P>0.05), until 21 and 28 days old, the number of the indexes began to gradually improved significantly.Conclusion: Adding AFB1 of 2.8 mg/kg to the diet can cause significant damage to liver and immune functions in chicken and adding 0.5 to 1 g/L of APS in drinking water can significantly relieve the damage of AFB1 to chicken. |
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