Studies On The Determination Of Adprin Residues By LC-MS/MS And Drug Elimination In Chicken Tissues

Adprin is a drug belongs to nucleotide compounds and be valid to tenella eimeria acervulina eimeria, maxima eimeria, and necatrix eimeria, which has been provided with high efficiency, broad-spectrum and low toxicity. However, it is necessary to monitor the drug residues of Adprin in order to make sure of the safety of animal food and the health of human beings. For the sake of building a more simple, rapid, sensitive and accurate analysis of Adprin, and also the domestic technical support of monitoring Adprin residues in chicken tissues, The study is committed to establish Adprin in chicken tissues detection method which is called high performance liquid chromatography-tandem mass spectrometry to promote the fuether study of the drug consumption in chicken.A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) for the determination of Adprin was established,and And the elimination rule of Adprin in chickens was studied. In the method, the samples of muscle,liver,kidney were extracted by methylenechloride concentrated by transpiration under 60 ℃, then the residue was resolved with 40% methanol,defatted with hexane and cleaned up with C18 cartridge;the plasma samples were prepared with C18 solid-phase extraction column.The separation of Adprin was performed on Agilent 1100 system with Luna C18 (2) column (5μm,150mm×2.00mm) and the elution solvent of methanol (containing 0.1% fomic acid) and water (containing 0.1% fomic acid) at a flow rate of 0.2 mL/min. The electrospray was operated under the positive ionization mode and the drug was identified under multiple reaction monitoring (MRM) mode. The method was quantified by the external standard method.The plasma and tissues samples were collected in different intervals after oral administration of Adprin, then detected by LC-MS/MS method. The concentration-time data of Adprin in plasma and tissues were analyzed with 3P97 pharmacokinetics software.The results showed that:the linear range was from 1 to 200 ug/kg (ug/L) with correlation coefficients (r2) more than 0.999. The limits of detection (LOD) were 0.6 ug/L、0.3ug/kg、0.6 ug/kg、0.3ug/kg, and the limits of quantification (LOQ) were 2.0ug/L、1.0ug/kg、2.0ug/kg、1.0ug/kg respectively in chicken tissuses. The average recoveries varied from 80.3%-94.9%. The intra-RSD were in the range from 2.2%-7.1% and the inter-RSD were from 2.2%-6.8%. The proposed method was simple、rapid、 sensitive and suitable for the quantitative determination and confirmation of Adprin.The results indicated the plasma drug concentration-time datas were two-compartment model with absorption. The absorption half-life(ti/2ka) of Adprin was 0.75 h, the elimination half-life(t1/2β) was 11.13 h. The time-point of maximum plasma concentration(Tpeak) and the maximum plasma concentration(Cmax) were 1.73 h and 184.98 ng/mL. The area under the concentration-time curve(AUC) was 1363.75 (ng/mL)·h.Tissue distribution revealed that Adprin in tissues were absorption in two-compartment model. The drugs were mainly distributed in the liver and kidney,the rate of absorption half-life(t1/2ka) was muscle<kidney<liver, the rate of distribution half-life(t1/2α) was liver<kidney<muscle,the elimination half-life(t1/2β) was kidney<liver<muscle.