Establishment Of Method For Determination About Adprin Content In Chichen By HPLC

Modern animal husbandry is the increasing tendency of large-scale and intensive production, the veterinary drugs were used to protect the normal evelopment of the healthy growth of livestock and poultry and livestock. It is easily lead to the unreasonable use of veterinary drugs residues in animal food. Not only affect the animal food safety, harm to human health, but also affect the international trade of animal products. In recent years, the nationwide outbreak of coccidiosis frequently, resulting in the unscientific use of anticoccidial drugs and a variety of efficient coccidiostat use of farmers to the economic interests of, resulting in livestock due to drugpoisoning occur and cause the veterinary drug residues in animal tissues has the potential carcinogenic and resistant to the possibility of residues of veterinary drugs in the human body.Adprin is a class of new anticoccidial drugs.which plays anticoccidial effect,interferes with purine metabolism of coccidian, is a broad-spectrum coccidiostat. Some researches were did by British scientists the seventies of the last century.It is a new class of effective anticoccidial drug in our country.It is necessary to establish a fast, accurate, efficient, wide applicability Adprin drug residue detection methods.Pre-treatment method of Adprin in chicken was established,and determined by HPLC method. Provided technical support for further research Adprin residue in the chicken.By HPLC chromatography system to determine the optimum detection wavelength and mobile phase selecti on study to determine the mobile phase flow rate and pH value, the best separation of chicken in Adprin drugs by adding methanol-water(50:50) mobile phase by ultrasonic extraction, column:XDB-C18,4.6*250MM,5μm.flow rate:1.0mL/min. detection wavelength:260nm. the column temperature was40℃;injection volume20μL, retention time qualitative and quantitative external standard method, the peak area. Themethod limit of quantification was25μg/kg. The linear range between100-500ng/mL linear correlation coefficient greater than0.9990. The method used for livestock determination of the recoveries of different concentrations were higher than70%, relative standard deviation≤10%, The method met the practical requirements of the analysis of samples.