Male Sterility Of Rice Physiological And Biochemical Research And Observation Of Pollen Cell Microfilament

Rice is one of the world’s most important food crops, currently about 50% of the world’s people with rice as the main food. Rice in China not only has a long history of cultivation, but also in food production also plays an important role. In China, rice plantings accounted for about 30% of all arable land. Its output accounts for more than 40% of all food crops production. One important reason is that rice male sterility and heterosis widely used.In this paper, Japonica male sterile line 451 A, maintainer line 451 B, 455 R restorer of research material to study the japonica male sterile line and maintainer line in the tillering and booting the lipid membrane peroxidation product malondialdehyde(MDA), superoxide dismutase(SOD), catalase(CAT) and peroxidase(POD) of dynamic changes. Also studied japonica CMS line 451 A and 451 B maintain the system during the development of pollen actin distribution, including the distribution of actin filaments within the cells of pollen, the pollen on the stigma of germination and pollen tube growth when microfilaments distribution. Through the study of Japonica male sterile material to obtain the following results:1.Rice vegetative stage of tillering stage sterile line leaves SOD, POD, CAT activity and MDA content showed abnormal, SOD activity is higher than the maintainer line CMS line, CAT activity but lower sterile line, suggesting that this anomaly CMS line leads to higher MDA content, but the change of MDA and three enzymes between CMS and the same trend line, so sterile line of membrane lipid peroxidation products accumulate more, while its normal metabolic system, with the the gradual accumulation of the damage may be a cause developmental stage after pollen abortion.2.Rice reproductive growth stages of booting stage by selecting pulvinus from labeling materials, measured SOD, POD, CAT activity and MDA content both in material selection panicle or in the blade material in the male sterile lines MDA content should be significantly higher than the maintainer line, and in which the third period- meiosis, MDA’s highest. And in the development process of young spike selected third stage- meiosis, male sterile lines contain higher SOD, CAT and POD activities, indicating increased and SOD MDA content in young panicle meiosis higher activity is an inductive effect. In order to study the results of experimental material leaves show activity of SOD in male sterile lines in stage three- meiosis highwhile keeping the activity of CAT and POD system were higher than CMS antioxidant capacity description leaves no Sterile Department weaker. Inferred in meiosis CMS in metabolic disorders may occur, significantly reducing the excessive accumulation of MDA and antioxidant enzyme activity, resulting in the generation of reactive oxygen balancing process is broken and cleared. Finally, because of metabolic disorders will affect the post meiotic pollen development, resulting in pollen in the process of development behind abortion.3.Observe the normal maintainer pollen spore mother cell stage development of small nuclear periphery and radially dense actin, actin visible rotating distribution.Meiosis I spindle showing bright fluorescence. Actin evenly distributed in the cell, in the late meiosis I, can clearly be seen in two stages with a large number of cells between actin filaments distribution. Meiosis Ⅱ in tetrad stage consisting of a dense network of actin filaments. Like the microfilament cytoskeleton to form a grid-like distribution in the cell, near the cell wall microfilaments perpendicularly oriented cell surface, the formation of three-dimensional three-dimensional network.4. Male Sterile Lines during the development of intracellular actin distribution exception occurs. Microspore mother cell meiosis, the formation of abnormal tetrads,some cells may appear without actin, indicating that a large number of actin in tetrad stage has disintegrated. Part pollen development occurs abortion case one nucleus period, when pollen is less inclusions intracellular actin observed a significant reduction or some cell deformability, but nuclear staining can be clearly better see.But also we observed that some pollen nucleus of abortion in the second, this time inside the cell nucleus and cytoplasm disintegration occurs, and finally into the pollen-free inclusions in pollen grains, the staining pollen can only see the outline and the nucleus.5. normal pollen grains of rice after staining was observed in pollen actin network distributed evenly in the cell. When pollen germination, the formation of polar actin distribution, a large number of actin accumulation and germination hole hole location away from germination part no microfilaria space. When the pollen tube growth,pollen tube was observed within a large number of microwires with pollen tube growth, pollen tube top there are signs microwires exist.In this study, Japonica male sterile line 451 A and 451 B maintain the system as material for its vegetative and reproductive growth during the course of some physiological and biochemical indexes were measured using a laser scanning confocalmicroscope for pollen development microfilaments distribution of the observational study, and as female sterile line 451 A, explore the distribution of Japonica CMS Line abortion during physiological and biochemical changes and pollen abortion process microfilament cytoskeleton. CMS lines for the production and use of hybrid breeding provides some practical guidance. In this study, to explore the physiological and biochemical and cytological sterile line sterile mechanism has some theoretical value and the molecular mechanism of their infertility remains to be investigated.