| Chinese chive(Allium tuberosum Rottler)is a typical cold resistance plant, but can not endure high temperature and light. It is traditionally been cultivated in open field under high temperature and light intensity, so it usually shows more crude fibre that lead to harder meat and bed taste. Focusing on these problems, the demand characteristics of Chinese chives to light quality was researched with ‘Ping Jiu 2’ as experimental materials. The light quality were white: red light=4:1(WR); white: blue light =4:1(WB); white: green light =4:1(WG); white: purple light =4:1(WP), using LED light sources. White light(W) treatment was used as the control. On this basis, the effect of color films on growth, yield and quality of Chinese chives were investigated. The main results are as follows:1. WR light increased the photosynthetic rate(Pn) and RuBP carboxylase(RuBPCase) activity significantly, compared with the control. WP, WG, WB treatments did not affect the Pn, but increased the RuBPCase activity markedly. The total sugar content of WR treatment was the highest, then WP treatment, and the WG and WB treatments were the lowest among the four treatments. WR treatment plants showed a significantly higher sucrose phosphate synthase(SPS) activity, whereas lower sucrose synthetase(SS) and amylase(AMS) activities than the control plants. WP did not affect the SPS and SS activities, but the AMS activity was significantly higher than that of the control. WB treatment showed higher SS and AMS activities, while lower SPS activity than the control. WG decreased the SPS and AMS activities, but no significant difference was observed in SS activity between WG treatment and the control. These data suggest that increased the proportion of red and purple light can improve the caron assimilation and transformation, and accelerate the sugar accumulation of Chinese chives.2. WB and WP treatments showed an significant increase in total nitrogen, protein nitrogen and non-protein nitrogen contents, and activities of nitrate reductase(NR), glutaminase(GS) and glutamate synthase(GOGAT), but a marked decrease in glutamate dehydrogenase(GDH) activities, in comparison with the control. WG treatment revealed an obviously higher total nitrogen and protein nitrogen contents, and activities of GS and GOGTA, while lower non-protein content and GDH activity. The protein nitrogen and non-protein nitrogen contents were lower in WR treatment plants than in the control plants. However, the GS and GDH activities were higher in WR treatment plants than in the control plants. These data indicated that blue, purple and green lights could promote the nitrogen metabolism, while red light might inhibit the protein synthesis in Chinese chives.3. WP and WR increased the plant height, pseudostem diameter, leaf width, leaf thickness, and single plant weight of Chinese chives significantly. WG treatment showed an increase in pseudostem diameter and single plant weight, but no remarkable differences were found in plant height, leaf width and leaf thikness between WG and the control. WB treatment inhabited thickening growth, and revealed a decrease in leaf thickness and plant weight of Chinese chives.4. The color films decreased the transmission ratio of visible light(400~700nm), while increased the transmission ratio of ultraviolet light, near-red light and far-red light. The red plastic greenhouse showed an increase in long wavelength light, however the blue plastic greenhouse revealed an increase in short wavelength light. The short wavelength light was reduced, but the long wavelength light had no obvouse change in purple plastic greenhouse. The green plastic greenhouse showed lower ratio of long wavelength light than the control, but no significant difference in the ratio of short wavelength light between green and control plastic greenhouse.5. The purple film treatment plants showed the thickest pseudostem, and higher single plant weight, indicating that purple film is beneficial to promote growth of Chinese chives. The yield and quality of the purpl film treatment was also higher than that of the control.6. The purple film by 65 ~77% light transmittance treatment plants showed higher Pn, Fv/Fm and ΦPSⅡ, yield, and better quality. So it can be used as the dedicated film of Chinese chives. |
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