| Stem blister canker, caused by Botryosphaeria dothidea, is becoming the most serious disease of poplar in China. This fungi distributes worldwide, parasites wide ranging hosts, and harms the branches and fruits of many kinds of forest and fruit trees. To reveal the global transcriptional changes of poplar to infection by B. dothidea, Solexa paired-end sequencing of cDNAs from control (NB) and pathogen-treated samples (WB) were performed, resulting in a total of 339,283 transcripts. Enrichment analysis and differentially expression were carried out, and miRNA target genes of this transcriptome were identified through bioinformatics analysis.The results of the study laid a foundation for mining the candidate genes associated with response canker. The results showed below:(1) 71629 of all the unigene sequences are subjected to 64 gene ontology annotation (GO).33304 genes were marked by KEGG functions annotations, and mapped to 319 function metabolic networks.(2) 206586 transcripts were differentially expressed in response to pathogen stress. In enrichment analysis, energy metabolism and redox reaction-related macromolecules were accumulated significantly in Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses, indicating components of dynamic defense against the fungus. Moreover, transcripts encoding proteins(e.g. glutathione S-transferase) were accumulated to high levels, revealing key genes and proteins potentially related to pathogen resistance.(3) Prediction of miRNA target genes was applied with psRNATarget using previously deposited miRNA sequences from Populus trichocarpa. A total of 772 target genes from 347 miRNAs were identified from Populus tomentosa under the infection to stem blister canker. Those genes encode proteins involved in disease-resistant categories, like plant hormone signal transduction, plant-pathogen interaction, glutathione metabolism, etc. |
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