Comparative Analysis Of The Differentially Expressed Micro Rnas And Transcriptomes In Response To Liver Regeneration In Chiloscyllium Plagiosum

The Chiloscylliumplagiosum（whitespotted bamboo shark） is known as a representative cartilaginous. The liver constitutes 75% of the viscera weight, which contains various biologicalfactors.The molecular mechanism regulating liver regeneration in C hiloscylliumplagiosumremains to be determined due to the lack of micro RNA and transcriptome profiling.onregenerative liver.The micro RNA and transcriptome of normal and 3 h regenerative livers were sequenced by Illumina Sequencing platform. 670 micro RN As were identified with 290 known micro RNAs and 380 PC micro RNAs. 339998 Unigenes were sequencedand used to scan five available databases.60047 Unigenes were confirmed by BLAST analysis.Differential expression profile ofmicro RNAs and Unigenes during liver regeneration processwould beinformative for further exploring the regulating network during liver regeneration.119 micro RNAs were found differentially expressed in 3 h regenerative liverafter partial hepatectomy. mi Randa and Targetscan were used to predict target genes from these 119 micro RN As.Theresulting candidate genes were then subjected to GO function enrichment analysis, which can be mapped to 28 classes, including “cell division”, “cell cycle” and “G2/M transition of mitotic cell cycle”. Total of 8 enrichment pathways were identifiedinluding “Cell cycle” and “Jak-STAT signaling pathway”. These 5 differentially expressed micro RNAs including four up-regulated micro RNAs and one down-regulated micro RNA, the Unigenes were all down-regulated.According to the target gene prediction software, the targeting effect between the three micro RNAs（PC-5p-13888₁68, xtr- mi R-34b_L-1R+1 and tni- mi R-144） and three Unigenes（IC A, GSTP1 and PDRX 5）was found. Then 3 ’UTR（3’ untranslated region） full- length sequence of the ICA, GSTP1 and PDRX 5 were cloned in psi C HECK2 vector, and at the same time, the binding site mutation sequences of these three 3 ’UTRs were also constructed. Thus getting recombinant plasmids, then transfected the huh7 liver cancer cells, and simultaneously transfect micro RNA mimics and psi C HECK 2 carrier, according to the different renal luciferase activity and SPSS software analysis, GSTP1 is the target gene of xtr- mi R-34b_l-1r+1（P<0.01）.In this study, The micro RNA and transcriptome of normal and 3 h regenerative livers were sequenced.Part of themicro RNAs and Unigenes,differentially expressed in normal and 3 h regenerative liverwere validated.Selective expression of micro RNAs and Unigeneswould shed light on the understanding of molecular mechanism regulating liver regenerationin Chiloscylliumplagiosum.