Illumina MiSeq Sequencing Analyzes Microbial Community Structure In Two Culture Models

Biofloc was a kind of reunion material formed by microbial community, plankton, organic debris and some polymer. Biofloc technology was one of the current advanced technologies. Microbes played a very important role. They were not only decomposers, but also consumers and producers in the culture water. They were effective to ecological system by transformation and adhesion function in the biofloc technology culture system. Microbial research methods were changing over time, which from the initial traditional culture to the current second generation sequencing technology. It made the results accurate and comprehensive. Microbial community structure in the biofloc technology culture system was studied through the high-throughput sequencing technology and Biolog-ECO.1. Abundance dynamics and community functional diversity of bacteria in Litopenaeus vanname biofloc technology culture systemThe abundance dynamics and community functional diversity of bacteria in biofloc technology(BFT) and traditional pond(Control) culture systems were investigated by agar plate assaying and Biolog-ECO microplate technology. The results showed: The number of heterotrophic bacteria in water increased at first and decreased in the later period. The amounts of heterotrophic bacteria in sediment fluctuated consistently with time. And it exhibited ascending tendency. The number of vibrio exhibited similar tendency in water and sediment. Two ponds were no significant difference. Early culture, microbes’ metabolic activity of carbon in BFT group was lower than Control group, but it was higher than Control group at the end of culture. Shannon, Simpson, and McIntosh index were BFT>Control. End of the culture, microbes’ carbon metabolism of polymers and amino acids was higher than other carbon sources in water and sediment. Microbes utilized aromatic almost zero in sediment, with significant preference.2. Illumina MiSeq sequencing analyzes Litopenaeus vanname in biofloc technology culture systemUsing Illumina high-throughput sequencing technology analyzed microbial community structure. The results showed that microbial community structure changed consistent. There were similar dominant bacteria species in water and sediment. Culture period had greater impact than culture system for the changes of microbial community structure. Microbial community structure was similar in the early culture and late culture, and it had significant advantage bacteria species. There were significant differences between it and mid-culture. At the phylum level: Bacteroidetes and Proteobacteria were advantage bacteria. At the class level: Sphingobacteria was advantage bacteria. At the genus level: Pedobacter was advantage bacteria.3. Abundance dynamics and community functional diversity of bacteria in non-situ biofloc technology culture systemThe abundance dynamics and community functional diversity of bacteria in situ(BFT situ) and different hydraulic retention time(2h, 4h, 6h) non-situ biofloc(BFT nonsitu) technology culture systems, were investigated. The results showed: The number of heterotrophic bacteria showed a rising trend. Microbes’ metabolic activity of carbon was rising in BFT situ group and HRT 4h group; HRT 2h group remained the same; HRT 6h group first decreased and then increased. There was significantly different of carbon metabolism at early culture, but it became consistent at the end of culture. Microbes’ carbon metabolism of polymers and amines was higher than other carbon sources.4. Illumina MiSeq sequencing analyzes microbial community structure in non-situ biofloc technology culture systemUsing Illumina high-throughput sequencing technology analyzed microbial community structure in non-situ biofloc technology culture system.The results showed: There were similar dominant bacteria species among different groups. At the phylum level: Fusobacteria, Proteobacteria and Bacteroides were advantage bacteria. At the class level: Fusobacteria and Alphaproteobacteria were advantage bacteria. At the genus level: Cetobacterium was advantage bacteria.