| The cryopreservation of rabbit can more time of preservation than preservation in low temperature but sperm cells have a high content of unsaturated fatty acids in their membranes,and sperm will be attacted by reactive oxygen species(ROS)which is produced with cryopreservation.ROS lead to lipid peroxidation, which would do damaged to the membrane of sperm, resulted in decreasing the motility of sperm and the rate of fertilization, and those would reduce the delivery rate and limit the widely-used of artificial insemination finally. The objective of the present study was to determine whether addition of cysteine to freezing extender could protect rex rabbit spermatozoa against cryodamage. The freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with different concentration of cysteine(0mM,2.5mM,5mM,7.5mM,10mM). The motility of post-thawed sperm was evaluated by visual estimation with an optical microscope, and kinds of sensitive fluorescent probes such as SYBR14/PI, FITC-PNA, JC-1,BODIPY 581/591C11 were used to asses of the membrane integrity, acrosome integrity, mitochondrial potentials and lipid peroxidation of the post-thawed sperm. The result show that spermatozoa with motility, acrosome intact, membrane integrity and mitochondrial potentials in the treatments was significantly higher than that of the control in supplementaion of 5mM and 7.5mM cysteine with freezin extender(P<0.05). Supplementation of 2.5mM or 10 mM cysteine with freezing extender show that spermatozoa with membrane integrity and mitochondrial potentials in the treatments was similar to tht control(P>0.05). The cryopreservation of rabbit in rabbit breeding industry recommend to supplementation of 5mM or 7.5mM cysteine with freezing extender. |
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