Molecular Marker Assisted Breeding Of Transgenic Herbicide-Resistant Lines In Rapeseed(Brassica Napus L.)

Rapeseed(Brassica napus L.)is one of the largest oil crops in the world, and is the first important oil crop in China. With the rapid development of China’s national economy, a large number of labors in rural areas have moved to the city. The mechanization and industrialization has become the main direction of the rapeseed industry. How to effecitively control weeds in rapeseed field has become the serious problem in our country, which seriously affected the rapeseed yield, quality and economic benefits. Comprehensive control of weeds is the key to the mechanization of rapeseed industry. It is one of the most effective ways to control weeds by using the herbicide-resistant rapeseed varieties. By using a Canadian rapeseed(B. napus) line C9 with glyphosate resistance which conferred by the gox gene, and the restoring line C5-1 and male sterile line C5-2 with glufosinate resistance which respectively conferred by the bar, barnase and barstar genes, as the donor parents, and Gr-1, Gr-2, two elite local rapeseed lines as the recurrent parents, the glyphosate resistance and glufosinate resistance was successfully transferred into Gr-1 and Gr-2 backgrounds by the combination of detection of the target genes( gox and bar) by PCR method and spraying herbicide solution method, and simple sequence repeat(SSR) marker assistant background selection. As a result, in BC3, glyphosate resistant plants with﹥96% genetic background recovery rates were successfully developed. And glufosinate resistant male sterile lines and restoring lines with﹥96% genetic background recovery rates were successfully developed. The main results of this study were as follow: 1. The glyphosate resistant gene gox was successfully transferred into Gr-1 and Gr-2, in BC3, glyphosate resistant plants with ﹥ 96% genetic background recovery rates were successfully developed. 2. The glufosinate-ammonium resistance gene bar, and barnase and bartar, were successfully transferred into Gr-1 and Gr-2, in BC3, glufosinate-ammonium resistant male sterile lines and restoring lines with﹥96% genetic background recovery rates were successfully developed.