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Analysis Of Nutrition Elements And Poisonous Components Of Five Kinds Of Poisonous Plants From Natural Grassland In Tibet

Posted on:2016-08-13Degree:MasterType:Thesis
Country:ChinaCandidate:X X LiuFull Text:PDF
GTID:2283330461966552Subject:Clinical Veterinary Medicine
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There are 365 kinds of poisonous plants belonging to 74 families 202 genera in Tibet’s natural grasslands. The distribution area of these poisonous plants is 10,733,000 hm2, about 19.51% available grassland area in Tibet. The stem Astragalus strictus, Oxytropis glacialis, Oxytropis kansuensis, Oxytropis serilopetala, Stellera chamaejasme, Ligularia rumicifoliaand Aconitum kongboense and other poisonous plants widely distributed in Tibet. Because of high density, the available prairie seriously reduced. Many livestock died as result of eating these poisonous plants, this is a serious harm for animal husbandry in Tibet.In this study, five poisonous plants flower early stage of flowering and fruiting of nutrients and toxic components were determined to clarify climate nutrients and toxic components of the dynamic changes of their different objects, providing the basis for the prevention and control of toxic plant science and rational utilization information. In this study, following results were obtained.1. 5 poisonous phytochemicals pretest: Test tube method with paper chromatography method are used for systemic pre-test for chemical composition from Astragalus Tibet, Gansu Oxytropis, hair flap Oxytropis, Stellera chamaejasme and aboveground reservoirs Ligularia. The results showed that: Astragalus contains protein, amino acids or peptides, saponins, organic acids, flavonoids, volatile oil and grease, phenols or tannin and alkaloids, excluding cardiac glycosides, anthraquinones and coumarin; hair flap spine beans contain protein, amino acids or peptides, saponins, organic acids, flavonoids, anthraquinone, phenols or tannin and alkaloids, excluding cardiac glycosides, volatile oil and grease as well as coumarin; Gansu Oxytropis contains protein, amino acids or peptides, saponins, organic acids, flavonoids, anthraquinone, phenols or tannin and alkaloids, excluding cardiac glycosides, volatile oil and grease as well as coumarin; Stellera chamaejasme contains protein, amino acids or peptides, organic acids, flavonoids, volatile oil and grease, anthraquinones, coumarin, a phenolic or tannin and alkaloids, excluding saponin, cardiac glycosides; Tibetan Ligularia contains protein, amino acids or peptides, saponins, organic acids, flavonoids, volatile oil and grease, quinones, phenols or tannin and alkaloids, excluding cardiac glycosides and coumarin.2. Analysis of the nutritional content of 5 poisonous plants: according to the national standard method, the conventional nutrients, mineral nutrients and amino acid from different physical possession of natural astragalus in Tibet, Oxytropis in Gansu, hair flap Oxytropis, Stellera chamaejasme and of Ligularia were analyzed. The results show that: in the five species, the average content of crude protein were highest in stem of Astragalus, Gansu Oxytropis followed; the crude protein content of them are more than 19%; the highest crude protein content is flowering stem in Astragalus,the lowest crude protein content is at preflowering period in Astragalus; the highest crude protein content is at pre-flowering period, the lowest crude protein content is at fruiting period. The average crude fiber content from the highest to the lowest is valve Oxytropis, Tibetan Ligularia, from Astragalus, Oxytropis beans and Stellera L., and the highest crude fiber content is at fruiting period, lowest crude fiber content is at pre-flowering period; the highest content of Mg, Fe, Cu, Mn and Zn is at preflowering period; the lowest content of Mg, Fe, Cu, Mn and Zn is at fruiting period; but Se relatively high content is at fruiting period. Total amino acids were 13.683%, 12.722%, 9.980%, 9.248% and 6.747% in Astragalus, Oxytropis Gansu, Stellera L., hair flaps and Tibetan Oxytropis Ligularia, relatively. total amino acids are highest in Astragalus, Oxytropis hair flap at flowering period, and is highest at Gansu Oxytropis and Stellera Euphorbia at preflowering period.3. Analysis of the toxic components of 5 poisonous plants: the total alkaloids and flavonoids from different physical possession of natural astragalus in Tibet, Oxytropis in Gansu, hair flap Oxytropis, Stellera chamaejasme and of Ligularia were analyzed used nonaqueous titration and fluorescence spectrophotometry. The results showed that: the average total alkaloid content was 1.016%, 0.581%, 0.310%, 0.263% and 0.251% in Gansu Oxytropis, from Astragalus, Stellera L., hair flaps and Tibetan Oxytropis Ligularia,relatively. total flavonoids contents were 0.607%, 0.508%, 0.442%, 0.475% and 0.478% in Gansu Oxytropis, from Astragalus, Stellera L., hair flaps and Tibetan Oxytropis Ligularia, relatively. total alkaloid content of Gansu Oxytropis is pre> flower> fruit period; the highest total alkaloid content in Astragalus, Oxytropis and Tibetan wool flap Ligularia is at flowering period. the highest total alkaloid content in Stellera chamaejasme is at fruiting period. Total Flavonoids is higher in Gansu Oxytropis at flowering and fruiting; the highest total flavonoids in Astragalus, Oxytropis hair flap, Tibetan Ligularia is at pre-flowering period; the highest total flavonoid content in Stellera chamaejasme is at flowering and fruiting period.
Keywords/Search Tags:Tibet, natural grassland, nutrition analysis, poisonous plant toxicity analysis
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