Drought Resistance Identification Of T5 Generation Transgenic Wheat Lines With The Y82 And W42 Genes Of Drought Resistance

Wheat is one of the major food crops and the drought is one of the important abiotic stress factors that influence the increase in production of wheat. So it is significant that we breed drought wheat varieties. The progress of conventional breeding drought is slow, but the transgenic technology has the short period, purpose and other advantages over traditional breeding methods.Therefore with the transgenic technology development and maturity, it provides a powerful way to foster resilience of new varieties of wheat in the short term. First, the experiment was conducted positive test for new strain in transgenic wheat of T5 generation by Y82/W42 gene. Among them, Y82 is a gene of receptor protein kinases and W42 is a gene of DREB transcription factor. After Obtaining positive plants, identify physiological and biochemical indexes and investigate agronomic traits. Final, analyze Drought resistance of transgenic wheat. This will provide a scientific basis for the transgenic breeding of the new and drought-resistant wheat lines.The major findings are as follows:1. The results of drought resistance identification of transgenic wheat lines by the Y82 gene show: the chlorophyll content, photosynthetic rate, relative water content of transgenic wheat lines that are X986,X1017,X1126,X1142 with control that is KE NONG 199 have been declining. The transpiration rate showes the first rise after falling trend and reaches peak in the flowering. The soluble protein, proline content, POD activity, SOD activity and relative conductivity continuously increase. The indexes of transgenic wheat lines are always greater than the control throughout the process. The agronomic traits of transgenic wheat lines that are X986, X1017, X1126 and X1142 have been higher than the control that is KE NONG 199. And transgenic wheat lines were respectively increased by 16%, 17%, 8% and 17%. The drought resistance indexes are 1.37, 1.35, 1.37 and 1.17. Among, the drought rank of X1126 is strong, and the drought Levels of X986, X1017, X1142 are very strong.2. The results of drought resistance identification of transgenic wheat lines by the W42 gene show: the chlorophyll content, photosynthetic rate, relative water content of transgenic wheat lines that are X557, X567, X560, X186 have a slower declining than the control that is JI MAI 22. The transpiration rate has a slower rising after a faster declining. The soluble protein, proline content, POD activity and SOD activity have a faster rising than the control. The relative conductivity has a slower rising than the control. The indexes of transgenic wheat lines are always greater than the control throughout the process. The agronomic traits of transgenic wheat lines that are X557, X567, X560 and X186 have been higher than the control that is JI MAI 22. And transgenic wheat lines were respectively increased by 13%, 14%, 15% and 13%. The drought resistance indexes are 1.28, 1.31, 1.34 and 1.29. Besides, the drought rank of X557 and X186 are strong, and the drought Levels of X567 and X560 are very strong. This shows that the drought resistance of transgenic wheat lines is stronger than the control.