Screening, Optimization And Related Low-temperature Fermentation Of Cellulose-degradating Microorganisms

National manure production has been reached 2.545 billion tons in 2011, as lack of effective management and processing application technology, manure has caused serious environmental pollution. On the other hand, according to the relevant information, the biomass produced each year is 5 to 10 times the total human energy consumption, which is rich in cellulosic materials. China is a large agricultural country, which are rich in cellulose materials not easily degraded. If we can make use of the two kinds of resources, that will be of great significance for increasing soil fertility, improving soil structure, reduce environmental pollution and promote high-yielding crop production.However, northeast, especially around Harbin, belonging to the alpine region. Cold fall and winter will greatly reduce the microbial activity, resulting in difficulty in starting compost and straw degradation, so we need to use relevant technology to improve the speed of starting compost at low temperatures to improve production efficiency and quality.This study takes the rural natural compost and paddy soils as a source, using conventional separation techniques, after screening and rescreening, we got the cellulose degrading bacteria DF3, DF8, DF25, they belong to Alcaligenes sp., Alcaligenes sp. and Brevundimonas terrae(T). Starch degrading bacteria T4, T10 and DF27, which T4 belongs to Brucellaceae bacterium KAV, Carbohydrate degrading bacteria DF26, belong to Pusillimonas sp. Then we use the co-culture(composite strains) methods to degrade cellulose. In order to get a higher enzyme production, we used response surface optimization method: take ino carbon, nitrogen, culation, p H value, surfactant content, fermentation time, rotation speed, temperature into single factor test, followed by Plackett-Burman experimental design, steepest ascent experiment and response surface methodology and related BBD validation tests, finally got the optimum conditions for enzyme production of DF3: inoculation is 8.9ml, p H value at 7.5 and a temperature of 40 ℃, nitrogen is 9.5g/L when the activity is 1848 IU, the optimum conditions for enzyme production of DF8: inoculation is 5.7ml, rotation speed is 105 rpm, carbon is 11.5g/L when the activity is 572 IU. Finally, after the solid-state fermentation,we put agents inoculated into compost, we found that the addition of agents into compost can quickly started.Compared with the control group, four days ahead of a high temperature period, the temperature reached 60℃ lasted at least 7d, the effect of the agents are very good. Then we study of applying different chemical fertilizers and organic manure produced on cabbage, and found after application of organic manure can improve soil humic acid content, compost product group acres yield about 9990 kg, fertilizer group of about 8702 kg, the control group was about 7361 kg. In order to combine a fibrous waste and animal manure effectively to alleviate the environmental pollution caused by livestock manure, improve the utilization of cellulose.