The Dandelion Water Extract On Biological Membrane In Vitro By Intervention Effect Of Streptococcus Suis

Streptococcus suis is a common infectious disease caused by Streptococcus suis, Streptococcus suis in the body easy to form biofilm, clearance of the immune system of the host resistance, can be persistent colonization, causing chronic recurrent infections, difficult to control. Dandelion is a common heat clearing and detoxifying herbs, the main active component for chlorogenic acid, with a broad spectrum of antibacterial anti-inflammatory effect. The dandelion extracts of Streptococcus suis in vitro intervention of dandelion to Streptococcus suis bactericidal effect and inhibition of biofilm formation and provide basis for dandelion disease prevention and control of Streptococcus suis and its mechanism of.Test method: take L9(34) orthogonal experimental method, respectively, Water and ethanol as the extraction solvent dandelion antibacterial effect as a reference, inspect water extraction liquid ratio, boiling time, boiling the extraction of three factors affect the number of antimicrobial active ingredients; the alcohol extract study solid-liquid ratio, ethanol concentration, p H on the extraction of three factors influence antimicrobial active ingredients, Ultimately determine the optimum extraction dandelion antibacterial ingredients. High antibacterial activity of extracted components chlorogenic acid as reference, measured by HPLC extract its chlorogenic acid content. Determination of dandelion extract and chlorogenic acid on suis minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) by broth dilution method, respectively. Application of biofilm forming device, using crystal violet staining(CV), using enzyme determination of Streptococcus suis biofilm formation ability of calibration by 1/2, 1/4, 1/8, 1/16, 1/32 MIC dandelion extract and 1/2, 1/4, 1/8, 1/16, 1/32 MIC of chlorogenic acid on Streptococcus suis were biological intervention experiment on Determination of two to form a membrane biofilm formation of Streptococcus suis were whether the intervention effect; using scanning electron microscope(SEM) detection of morphological effect of water extract and chlorogenic acid in dandelion the biofilm formation of Streptococcus suis.Test results: the L9(34) orthogonal experimental method and optimization of dandelion extract inhibitory effect on Streptococcus suis the best extraction technology, the optimum extracting technique of dandelion is solid to liquid ratio of 1:15 and decocting time is 30 min, the times of decoction for 3 times, this group of Pseudomonas chlorogenic acid content 5.833 mg·m L-1. The matter of Streptococcus suis by minimum inhibitory concentration(MIC) and the minimal inhibitory concentration(MIC) were 62.5mg·mL-1 and 250mg·ml-1 dandelion water. The minimum inhibitory concentration of chlorogenic acid on Streptococcus suis(MIC) and minimum bactericidal concentration(MBC) respectively, and 2.5mg·m L-1and 10 mg·m L-1. Scanning electron microscope observation of visible negative control group of bacteria embedded in the biofilm, bacteria surrounded by thick mucus layer, and dandelion water provided and chlorogenic acid can make bacteria were membrane in the number of bacteria and biological membrane had obvious changes in morphology and significantly reduced the number of bacteria, no mucus and extracellular matrix, the second to Streptococcus suis organisms are membrane formation were inhibited, showed a dose dependent.Conclusion: Dandelion water extract on biological Streptococcus suis in vitro biofilm formation has an inhibitory effect, and showed a dose-dependent; dandelion water extract on biological Streptococcus suis in vitro biofilm formation inhibitory effect of the main active ingredients chlorogenic acid.