Collection Of Pseudomonas Syringae Pv. Actinidiae Strian And Their Hosts And Vectors Identification

Kiwifruit (Actinidae spp.) initially originating from China, enriching Vc, is entitled "The king of fruit". Kiwifruit bacterial canker (KBC), caused by Pseudomonas syringae pv. actinidiae (Psa), is the most serious destructive bacterial disease on the production of kiwifruit. Our country and the European Plant Protection Organization (EPPO) respectively listed Psa as forest phytosanitary objects and A2 class phytosanitary pests to prevent and control the occurrence of disease spread. The disease mainly harms leaves, vines and branches, leading trees to being weak and death even to ruin the whole orchard.The paper mainly identified and collected Psa strians their potential vector as well as non-Actinidae plants from the occurrence of KBC in the major production areas..The main research results as follows:1. A total of 223 Psa strains were collected from diseased tissues of Actinidia cultivars (Jinfeng, Guichang, Hayward, Hongyang), stock in our china major kiwifruit producing areas, including Yuexi and Jinzhai County, Anhui Province, Huxian and Meixian County, Shaanxi Province, Sichuan Mianzhu and Pengzhou City, Sichuan Province, Xiuwen County, Guizhou Province and Jiading District, Shanghai City.2. A total of 10 Psa strains were collected from leaves tissues of non-Actinidia plants, including Setaria viridis (L.) Beauv., Alternanthera philoxeroides (Mart.) Griseb. and Paulownia tomentosa (Thunb.) Steud. The results showed that above plants were suitable inter-host.3. By using isolation and transmission capacity analysis, the results showed that Bothrogonia ferruginea possess the ability of spreading KBC.In conclusion, our studies collected Psa strain of each kiwifruit production area and showed that KBC existing interhosts and vectors. This study provided a theoretical basis of disease cycle and epidemic regularty of KBC and material for a further study.