Effects Of Intrauterine Growth Restriction During Late Pregnancy On The Growth And Development Of Kidney In Mongolia Ovine Fetus

This study investigated the effect of intrauterine growth restriction on the growth and development of the kidney of ovine fetus during late pregnancy. Moderate body condition, healthy Mongolian sheep with 2-3 parities of 24, using estrus synchronization to make them in pregnantion at the same time. At 90d of gestation, six ewes were slaughtered, and the remainding were randomly assigned to three different energy feeding levels groups:CG group (Free,0.67 MJME·BW-0.75·d-1); RG2 group (0.33 MJME·BW0.75·d-1); RG1 group (0.175 MJME·BW0.75·d-1), followed by lower energy levels. At 140d of pregnancy, all the remaining ewes were slaughtered as before. The results indicated:(1)With the increasing of the dietary energy density restriction degree during late pregnancy, fetal kidney weight and kidney growth rate were decreased significantly in RG2(P<0.05), RG1 (P<0.01)groups. The fat and DM in fetal kidney were significantly decreased in RG2(P<0.05), RG1 (P<0.05)groups. The water and protein in fetal kidney were signif- icantly decreased in RGl (P<0.01)groups. The ash in fetal kidney were significantly decreased in RG1 (P<0.01) and RG2groups (P<0.05).(2) With the increasing of the dietary energy density restriction degree during late pregnancy, the number of glomeruli/HPF were significantly increased in RG1 (P<0.05)groups. The structure of kidney was mild mesangial proliferation, increase the number of mesangial cells, thickening of capillary loops. Masson staining of collagen fibrosis showed a deepened trend, RGl group Ⅰ collagen concentration was significantly lower than the CG group (P<0.01).(3) With the increasing of the dietary energy density restriction degree during late pregnancy, the total DNA content in fetal kidney were significantly reduced in RG2, RG1 groups when composed to CG group(P<0.01).(4) With the increasing of the dietary energy density restriction degree during late pregnancy, the T-AOC concentration was lower(P<0.05),and MDA content was higher(P<0.01) in RG2, RG1 groups than those of CG group, in addition, the SOD activity was decreased(P<0.05) and the GSH-Px activity was increased(P<0.05) in RG1 group; the inhibit hydroxyl radical capacity of RGl groups was higher than CG group (P<0.01); the XOD activity of RG2(P<0.05) and RG1(P<0.01) group was lower than CG group; POD activity of RG1 (P< 0.01) group lower than CG group.(5) With the increasing of the dietary energy density restriction degree during late pregnancy, RG1 fetal kidney Scr and BUN was lower than CG, RG2 group (P<0.01); RG1 group fetal kidney β2-MG was significantly higher than CG, RG2 group group (P<0.05); RG1 group fetal kidney the Cys C was significantly higher than in the CG group (P<0.01).