Screening Genes Related To High Content Of Carnitine In Skeletal Muscle In Cashmere Goat

L-Carnitine is an essential nutrient, and can be used as healthy weight loss products. Inner Mongolia Cashmere goats has higher carnitine levels than other animals, and there are almost no research on carnitine content in different tissues, and no one knowns which gene(s) can influence on camitine levels. To find out the key gene(s) will be of great importantance in future carnitine study, mutton finely cutting and its sales. In this study, Inner Mongolia cashmere goat is took as the experimental animal model, measured total carnitine content of different muscle tissues by high performance liquid chromatography (HPLC), and we used RNA-Seq sequencing for the quadriceps femoris, biceps femoris and heart transcriptomes, that different parts and type of muscles have different levels of carnitine. Then we performed GO functional annotation and KEGG pathway analysis for the differentially expressed genes, and combined carnitine-related genes with the differentially expressed genes to identify the high carnitine content candidate genes in Cashmere goat skeletal muscle. It can provide theoretical basis and insight into further research on high carnitine content of cashmere goat. It can provide possiblity to maximize utilization of cashmere goats and create greater profits to enterprise. The main findings of this study are as follows:1. The total carnitine content of twelve different muscles of Cashmere goat were determined by HPLC. The skeletal muscles’ carnitine content(1217.53±14) are all higher than the cardiac muscle(446.45±33). In the skeletal muscle, the semimembranosus has the highest carnitine content, the quadriceps femoris has the second highest content, biceps femoris has the lowest content. In general, the carnitine levels of these muscles is that the hind leg muscles> shoulder-back muscles> boneless picnic shoulder muscles.2. We successfully constructed biceps femoris, quadriceps femori and heart transcriptome libraries of Inner Mongolian cashmere goat, based on RNA-Seq high-throughput sequencing technology. We totally get 7.2GB transcriptome data. Through the TopHat software for analysis, the biceps femoris and quadriceps femoris differentially expressed genes are 1864; biceps femoris and heart differentially expressed genes are 993; quadriceps femoris and heart differentially expressed genes are 2770.3. GO functional annotation of differentially expressed genes results showed:The differentially expressed genes are mainly related to muscle development, glucose metabolism, ATP metabolism, oxidative phosphorylation, ubiquitination, vesicle-mediated transport and mitochondria; KEGG pathway analysis showed that:The differentially expressed genes are primarily involved in PPAR signaling pathway, insulin signaling pathway and MAPK signaling pathway.4. Combined with carnitine-related genes on Geneontology website, the PDZK1, CDV3, ACADL, PRKAA2, PRKAB2, MOBAT1, NAT6, PLA2G15 and HRASLS might be the candidate genes of high carnitine content in cashmere goat skeletal muscle.5. Phylogenetic analysis shows:PDZK1, CDV3, ACADL, PRKAA2, MOBAT1, PLA2G15 and HRASLS in Capra hircus, Ovis aries and Bos taurus, Rattus norvegicus and Mus musculus have higher homology and conservation; Capra hircus’ PRKAB2 have lower homology and conservation compared with other species; Gallus gallus’PDZK1, CDV3, PRKAA2, PRKAB2, MOBAT1, PLA2G15 and HRASLS have lower homology compared with other species.