| ObjectiveThe survey about parasites endemic areas department of wild and farmed main economic fish near the sea in western of Liaoning Province, for the relevant departments to draw fish parasitic disease prevention and control measures to provide a reference.MethodsIn different seasons, has divided into several batches buy fresh dead fish or fish, from Panjin, Jinzhou, Suizhong 3 Fish Port and 9 coastal fish farms.Random sampling of 19 kinds of major economic fish, totaling 2803, of which 5 kinds of fishes 746,14 kinds of wild fish 2057.First,Fish species identificated, record the number and indicate the source and origin.Then fish parasitology systemic autopsy method to collect all the parasites in vivo.By visual observation and microscopic morphology classification and identification, and statistics infection, infection intensity, flora distribution.An unknown insect species of Eoacanthocephala and an unknown insect species of Trematoda respectively 18 Sr DNA gene sequence analysis to identify its species.ResultsThe survey found a total of 19 kinds of fish the waters of western Liaoning internal and external parasites infect 15 species belonging to 11 families and 15 genera, 7 classes.On the basis of statistics on the prevalence and intensity of infection, identify strengths insect species Anisakidae larvae Section 3 genera,Hysterothylacium,Contracaecum,Phocanema wild and farmed fish have a higher infection rate.And for four kinds of fish farming is concerned, in particular, flounder and turbot, its advantages genus Cryptocaryon irritans and Paralembus digitiformis,have a higher prevalence in various fish farms and morbidity.Acanthocephalan new insect species gene sequence analysis by 18 Sr DNA identified as B.neoechinorhynchus,Trematoda new insect species gene sequence analysis method has been 18 Sr DNA identified as Stomachicola muraeneocis.Conclusions1.The survey found a total of 19 kinds of fish the waters of western Liaoning external parasites infect 15 species belonging to 11 families and 15 genera, 7 classes.Advantages of insect species of fish parasites in western Liaoning waters are:Larva of Hysterothylacium,Larva of Contracaecum,Larva of Anisakis.2.The study found that a new species of neoechinorhynchus in Harengula zunasi and Symechogobirs hasta of western Liaoning and named BH-Liaoning neoechinorhynchus.Stomachicola muraeneocis founded live in the body of Muraenesox cinereus.Its first recorded 18 Sr DNA whole genome sequences. |