The Analysis Of The Microbial Community In Traditional Chinese Medicine Compound Additives And Fermentation Bed Mode

In this paper,the experiment is in Guilin,Xing Mu aquaculture Limited fermentation bed demonstration base. Mainly carries on the pig farm the transformation construction, whether does the traditional Chinese medicine feed additive can replace the antibiotic feed additive preventing and controlling pig Mycoplasmal Pneumonia of Swine, analysis the pHysicochemical property of fermentation padding and the main flora of the fermentation bed. The Escherichia coli (E.coli), StapHylococcus aureus (S.aureus), Salmonella(S.nella), Streptococcus suis (S.suis), Mucinous Bacillus subtilis (Mucinous B.subtilis), Bacillus subtilis (B.subtilis) as the main bacteria fermentation padding. The Escherichia coli, StapHylococcus aureus, Salmonella and Streptococcus suis are pathogens. Mucinous Bacillus subtilis and Bacillus subtilis are the probiotics. In the prevention of Mycoplasmal Pneumonia of Swine test, Chinese medicine rhubarb, that epHedra, Chai Hu, and the cycle, the Soviet Union, and winter flowers, and white legal system, and the semi-Mr Ronald ARCULLI, Mr Shum, licorice root, root and wood of different medicine.ith the suitable fermentation compound prescription Chinese native medicine black aspergillus which screens has planted carries on the fermentation degeneration, After the degeneration Chinese native medicine and western medicine originally only separately mix into the pig only foundation date grain. Observed and recorded incidence of swine Mycoplasma pneumonia. The result proved that, after the compound prescription Chinese native medicine mixes into the foundation date grain oral administration pig only, may promote the pig only to digest the absorption, the promotion pig effectively only grows, strengthens its immunity, reduces the disease incidence rate and the mortality rate. 1. Combining the advantages of other pig fermentation bed mode and traditional cement pig raising method, according to the climate conditions in South China, project a kind of fermentation bed with thin padding. Compared with the traditional method of pig feed, index including feed consumption and feed conversion ratio, water consumption, harmful gases (HiS、NH3). The results show that 3 batches of the traditional pig feed meat significantly and fermentation bed house have significant difference (P<0.05), fermentation bed house has low feed conversion ratio. ADG of pigs between two hog house have differ significantly significant difference (P<0.05), ADG of pigs of fermentation bed house is higher.3 batches of fermentation bed pig slaughter rate was 100%, while the traditional pig slaughter rates were 95%,93.3%, 92.7%, although the two groups had no significant difference (P> 0.05), but there is significant difference among groups (P<0.05). During the conservation period and fattening period, the content of two kinds of hog house of harmful gas H2S,NH3 is very significant (P<0.01), The concentration of harmful gas in fermentation bed house is much lower than the traditional content of pig. Thin padding method of fermentation bed pigs can not only solve the shortcomings of traditional method of pig and but also can achieve the effect of ecological pig feeding, is a new model of sustainable development.2. The depth of 15cm of fermentation bed padding is at low pH, high temperature, has a higher microbial carbon and nitrogen, and has a significant difference (P<0.05) with others. The content of nitrate nitrogen is high, with moisture content is low. It shows that depth of 15cm as the core area of microbial fermentation. The separation of bacteria Traditional medicine feed supplement fermentation bed padding, common feed supplement fermentation bed padding and The traditional pig healthy swine feces are roughly the same, mainly for Escherichia coli, StapHylococcus aureus, Streptococcus, Salmonella, Bacillus subtilis and viscous Bacillus subtilis. the content of Escherichia coli in the three groups are the same, the difference was not significant (P>0.05). The difference of Salmonella is significantly (P<0.05). The content in the TCM group is fewer than in the normal group. The content of the three groups of StapHylococcus aureus are less, the TCM group and the normal group had no significant difference (P>0.05), but fermentation bed padding and pig excrement had a significant difference (P<0.05). The content of Bacillus subtilis and Bacillus in Pig manure was very difference (P<0.01) with others. Especially viscous bacillus was little. The experimental results show that only the mouse pathogenic Bacillus subtilis and viscosity on mice with lethal. The results of drug sensitivity test showed that drug resistance of Escherichia coli, StapHylococcus aureus and Streptococcus suis broad spectrum resistance, Salmonella spectrum is not widespread.3. For investigating the protective effect of compound traditional Chinese medicine in preventing mycoplasmal pneumonia and the feasibility of substituting TCM for tiamutin as the drug for preventing mycoplasmal pneumonia.In this paper, we selected the compound TCM and Tiamutin as the drug for preventing mycoplasmal pneumonia which respectively added to pig diets according to the proportion.In TCM group, the incidence of 4 month were 16.67%,10%,6.67%,3.33%. And the incidence for Tiamutin group were 13.33%,10%,20%,26.67%. The experimental results show that, by using compound TCM to prevent and treat mycoplasma pneumonia of swine, can significantly reduce the incidence rate of mycoplasmal pneumonia of swine, relative to the Tiamutin group, with the passage of time, the control effect of compound TCM has been maintained, and control effect of Tiamutin group was getting worse. Therefore, the compound traditional Chinese medicine can replace of tiamutin to prevention and treatment of mycoplasmal pneumonia of swine. The incidence of three prescriptions in TCM group were 10%,10%,0%,0% and 20%,10%,10%;0% and 20%,10%,10%,10%.It shows that the first prescriptions works the best.