Study Of Identification And Classification Of Aquatic Diptera Insects On The Epidemic Area Of Camels’ Onchocerciasis In Alasan Of Inner Mongolia

In order to study the species and distribution of aquatic Diptera insects, the possibilities of each species being the intermediate host of Camels’ Onchocerciasis, and understand their interspecific relationship by the molecular systematical way, it was carried out that specimen collection and investigation of aquatic Diptera insects distributing in the camel living area in the desert steppe of Inner Mongolia in 2014. Firstly, species were determined by traditional morphological classification method; Secondly, the COI gene were amplified by the consensus primers(LCO1490、HC02198), followed by the gene sequenceing; Lastly, molecularly phylogenetic tree was constructed according to the result of a homology comparison and analysis by the Biosoftware DNASTAR.Morphological identification results showed that the specimen belonged to 49 species of 42 genera of 21 families, including 6 families unidentified. Among the identified aquatic Diptera insects, there were 18 unrecorded new species. Statistics results showed that Paracladius ovatus was the dominant species in all aquatic Diptera insects living in the desert steppe in Alashan Left Banner, Alashan League of Inner Mongolia, with a proportion of 12.92%. Among all the aquatic Diptera order, Aristocera, Nematocera and Brachycera, accounted for 45.22%,42.89% and 11.89% respectively, with Psilephydra cyanoprosopa, Paracladius ovatus and Gymnopternus shimentaiensis as their dominant species in the corresponding order.Molecular taxonomy results showed that the similarities of the COI sequences of the 12 aquatic Diptera insects ranged from 75.9% to 97.3%. Significant differences existed between different families of the same order as well as different genera of the same family. Through the research above, basic knowledge of the species composition of aquatic Diptera insects in the camel living area in Inner Mongolia were acquired, which would serve as data base for the subsequent research of the identification of the vectors of Camels’ Onchocerciasis, as well as important information for the research of the genetic relationship and the phylogenetic tree of aquatic Diptera insects.