Study On The Somatic Embryogenesis And Rapid Propagation In Spathiphyllum Inflorescence

In this study, Spathiphyllum inflorescence as the test material, carry out the direct induction of inflorescence somatic embryos and researching on rapid propagation, observation period, selectting the most effective solution for somatic tissue culture ang rapid propagation.The mian findings are as follows:1.The somatic embryogenesis in Spathiphyllum inflorescence1.1 In the test of Spathiphyllum inflorescence sterilization, using 3 different development stages of Spathiphyllum inflorescence as experimental materials, with 0.1% HgCl2 solution for different time, selected the optimal sterilization time at different developmental stages.The results show that, the highest sterile rate of the inflorescence obtained is 64.44%,the optimal sterilization time is 100min.1.2 Study on the direct induction of inflorescence somatic embryos, by 6-BA, KT and NAA orthogonal analysis, to screen out the optimal hormone combination is 6-BA 5.0mg/L+KT 1mg/L+ NAA 0.lmg/L, at the same time,through the analysis of range comparison the hormone,the results show that the 6-BA effect on the induction of somatic embryos most significant.Using two factors repeated the experiment, research on different development stage and different parts the induction of somatic embryos, the results show that the morphology of flowering at the lower end of the somatic embryo induction rate is the highest,inflorescence flowering were used as explants to induce optimal embryo.By the 3 levels of single factor experiment, to explore the influence of photoperiod on somatic embryos induction, finding the optimum culture condition for somatic embryogenesis is the first dark after 15 days of culture to light dark ratio as of 12:12.2. Study on rapid propagation technology in Spathiphyllum2.1 In research of the synchronized proliferation,using the single factor experimental design for screening medium for the best synchronized proliferation,6-BA 5mg/L+ZT 0.3mg/L+NAA 0.2mg/L is the optimal synchronized proliferation in Spathiphyllum inflorescence.By single factor experiment design,development of sucrose and glucose culture experiments,finding when the sucrose concentration is 45g/L,the glucose concentration is 30g/L,the proliferation of somatic embryos achieve the best effect.In the following study of the influence of generation frequency, the best subculture cycle control in 30-40d.2.2 On cultivating the inflorescence somatic embryos germination, a comprehensive comparison of hormone,activated carbon, sucrose and other factors, explore the best scheme of Spathiphyllum inflorescence culture of somatic embryos germination.The test results that IBA 0.1mg/L+GA3 0.4 mg/L+sucrose 30g/L is the best somatic embryo germination of hormone combination.When adding 6g/L activated carbon, therate of somatic embryo germination increase by 4.80%.2.3 In test of Plant regeneration transformation system,through the 6-BA,IBA and GA3 analysis orthogonal transformation,screening the best culture medium of plant regeneration is 6-BA 0.5mg/L+IBA0.1 mg/L+GA3 0.5mg/L,plantlet conversion rate as high as 88.56%.6-BA 1mg/L+IBA0.2mg/L+GA3 0.3mg/L is the optimal bud reproductive hormone combination, bud propagation coefficient reached 3.71. 3.Study on plantlet transplanting of Spathiphyllum and flower stimulationThe test results of the seedlings in vitro show that after seedling 6 days then opening the cover 2 days,transplanted to the tray covered with plastic film 14 days,the Spathiphyllum plantlets survival rate is 94.44%.Different concentrations of GA3 acid on flower stimulation of Spathiphyllum..Research shows that when GA3 concentration reaches 250mg/L, the most effective of flower induction,flower induction rate reached at 66.67%.