Immunity- And Reproduction-related Gene Expression Analysis In Locusta Migratoria

Locusta migratoria is the worldwide agricultural pests and harms our country agriculture seriously. Blaesoxipha lapidosa is a kind of locusts parasitic fly, which can parasitize many kinds of locusts. Research on the trade offs between immunity and reproduction is contributing significantly to the understanding of the fitness of organisms in nature. Focusing on the parasitism of Locusta migratoria by Blaesoxipha lapidosa, whose immune and reproductive systems are simple, facilitates such a study.The development of next generation deep sequencing technology has made tremendous contributions to the study of these biological processes at the molecular level. The transcriptome and DGE analysis of the fat body and ovary of L. migratoria based on the Illumina short read sequencing technology, de novo assembly and Cufflinks assembly. We assembled 54,740 non redundant unigenes using the Trinity program and 38,181 unigenes using the Cufflinks program. And all unigenes have been annotated.We performed a total of 12 analyses of differential gene expression in locusts that were parasitized by introducing Micrococcus luteus, Blaesoxipha lapidosa, or dextran beads(simulated parasite) into the hemolymph based on one transcriptome generated for the fat body and ovary tissues collected at 4 hours and 6 days post treatment. We identified energy, immunity and reproduction related genes in the 12 DGEs, that is hexamerin like protein about energy, GNBP(gram negative binding protein), PGRP(peptidoglycan recognition protein), lectin and apolipophorin 3b about recognition. Lm diptericin, serine proteinase, phosphoserine phosphatase, serpin precursor, serpin, pro phenoloxidase, phenoloxidase subunit A3, lysozyme, cytochrome P450, tolloid like protein 2, protein toll, toll like receptor about immunity, juvenile hormone, vitellogenin about reproduction. The results of our analysis of the differential expression of immunity and reproduction related proteins are the bactericidal effect of L. migratoria will reach the peak with 4 hours parasitism of M. luteus, which is quicker than encapsulation and L. migratoria will reduce Vg gene(instruction gene of reproduction) expression to redistribute energy when facing to immune challenge.