The Influence On The Immunocompetence And Physiological Property Of Locusta Migratoria Manilensis(Meyen)Parasitized By Blaesoxipha Lapidosa

Blaesoxipha lapidosa, an endoparasitoid fly that parasitizes Locusta migratoria manilensis (Meyen) is one of the important enemy insects and can feed with hemolymph,fat and muscle tissue in the chest and abdomen of the locusta. Locusta migratoria manilensis (Meyen) was used as research object in this paper and parasited by the larve of Blaesoxipha lapidosa artificially, so as to explicit the influence of parasitism on its immunocompetence. hemolymph proteome and respiratory metabolism. The main results present as follows:1, Several major immune factors of Locusta migratoria manilensis were studied at the different time after eclosion. First, four kinds of blood corpuscles including prophemocytes,granulocytes,plasmatocytes and oenocytoids were observed by using neutral red staining. Second, the phenoloxidase and lysozyme activity in haemolymph of the locusta was found to decrease remarkably at the sixth day after exclosions, which may be relavant to its sexual maturity under laboratory conditions.2, The change of immunocompetence in haemolymph of Locusta migratoria manilensis (Meyen) parasited by Blaesoxipha lapidosa was studied. The results indicated that the phenoloxidase activity was increased significantly at the first day after parasitism, which was decreased remarkably followed. Meanwhile, the lysozyme activity was increased at the first and second days after parasitism, but there was no significant difference followed.3, The alteration of hemolymph proteome in Locusta migratoria manilensis (Meyen) parasited by Blaesoxipha lapidosa was studied with2-dimensional electrophoresis, and the results showed that the protein abundence increased obviously.4, The alteration of respiratory metabolism rate of Locusta migratoria manilensis(Meyen) parasited by Blaesoxipha lapidosa was studied. The results showed that the respiratory metabolism rate was dereased obviously during1-3days after parasitism. Meanwhile, the rate of male individual was altered more apparently after parasitism because of its smaller body.