| Lipometabolism are complex traits controlled by multiple genes. Currently, most researches oflipolysis focused on a few or even a single gene. This method was difficult to detect interactionsbetween genes and had some limitations when applied to breeding practices. HSL plays an importantrole in lipolysis. Studies have confirmed that CBI could affect the expression of HSL gene, but theregulatory mechanism was not clear. After literature mining, this study not only constructed genepathways network which lipolysis related to, but also clarified the mechanism between lipolysis genes.This study speculated that CB1regulates HSL through the pathway of CB1→PPARγ2→perilipinA→HSL. We validated the regulation of CB1to the gene expression of HSL by testing. It could provide atheoretical basis for further research of lipolysis pathways in the future.This study analyzed the characteristics of the relevant enzyme and its encoding gene in threeaspects: fat mobilization, β-oxidation of fatty acids of mitochondrial and peroxidase, glycerolmetabolism. We also explained the mechanism of the regulation pathways. This study analyzedphysicochemical properties and structural genes of4proteins encoded by genes inCB1→PPARγ2→perilipinA→HSL pathway by bioinformatics methods. The experiment selected15pigs to sampling, which were4,5,6,7-month-old belong to U.S. department. It determined the relativequantification of gene expression of CB1, PPARγ2and HSL. The results showed that: excitatory Gprotein-coupled receptor pathway, inhibited G protein-coupled receptor pathway and Gq/PLC/PKCpathway belong to G-protein-coupled pathways, the regulatory mechanisms were similar; tyrosinekinase receptor pathway and JAK-STATs pathway belong to enzyme-coupled receptor pathway, whichhad the same regulation characteristics. Insulin pathway and JAK-STATs pathway are mutualindependent of adrenergic pathway. cAMP/PKA pathway and PKC/ERK pathway are independent ofeach other. Prilipin A and HSL were the terminal adjustment points of multi-pathway, and the two genesplay an important role in lipolysis.4proteins do not belong to membrane protein, and they without thesignal peptide. CB1and HSL was significantly positive correlation in liver, the correlation of two geneswas not significant in the omentum, longissimus dorsi, perirenal fat and backfat. The expression ofPPARγ2and HSL was positively correlation in omentum, longissimus dorsi, perirenal fat and backfat.The results show that it is a complex regulation mechanism from CB1to gene expression of HSL,and there may be multiple ways simultaneously. It may exist lipolysis pathway in pig fat tissue which the positive regulation to gene expression of HSL by PPARγ2mediated. |
PDF Full Text Request