Bovine Viral Diarrhea/Mucosal Disease And Serological Survey Isolation

Taken450serum and anus swab samples randomly from different areas in XinJiang，then did BVDV antibody and antigen detection through Double antibody sandwich ELISAand Double antigen sandwich ELISA.The results showed that163serum samples of BVDVantibody positive rate was36.2%;209serum samples of BVDV antigen-positive rate of46.4%.Centrifugd the positive anus swab samples of BVDV antibody and antigen by12000rpm at4℃for10min,the supernatant was sterilized by filtration, inoculated in bovinekidney cell monolayer covered（MDBK）.After5-7generations blind passage, the cellswere stored at-20℃, then melt at room temperature, Frozen and thawed three times，subpackaged the collection of cell suspension and stored at-80℃.The results show that theblind passaged cells were two variations: one was cell disease, another was not.Identifed the cells inoculatied are infected with BVDV by RT-PCR according to thecharacteristics of BVDV nucleic acid;extractied cell RNA, and then reverse transcribedinto cDNA according to conservative and specificity of BVDV5’-UTR regionsequences,did PCR amplification of the target bands by Referenced to the primer aboutrelevant literature.The results show that no amplified the target bands.This research has made the basic investigation of parts of Xinjiang BVDV serologicalprevalence and learned more about the virus infection and its prevention and control inXinjiang provides experimental data.