Research On The Isolation And Biological Characteristics Of Bovine Viral Diarrhoea/mucosal Disease Virus

In order to isolate and identify BVDV strains of Xinjiang,grasp the biological characteristics of the strain. Gathering feces which suspected as bovine viral diarrhea(BVD) in the northern parts of Xinjiang.Detected by RT-PCR,cell isolation and culture,indirect immunofluorescence test,immune electron microscopy and serum neutralization test for the separation and identification of BVDV. After the determination TCID50 of strain,and then the strain test for anti-ether、chloroform test antitrypsin test、acid test、the temperature sensitivity of the test and the nucleic acid typing tests to detect physical and chemical properties.By RT-PCR diagnostic samples appeared fragment at 286 bp. Select positive samples by RT-PCR,inoculated at a density of approximately 80% monolayer MDBK cells appeared cytopathic, Blind five passages to appear typical cell disease, the F5 cell cultures were used indirect immunofluorescence detection,resulting in a yellow-green fluorescence and same with C24V;By immune electron microscopy to see a lot of Spherical BVDV particles,about the size of 20-40nm; Serum neutralization test no cytopathic. Comprehensive five methods identified as BVDV strains.The result of TCID50 is 10-4.5,physicochemical assays showed that this virus was sensitive to chloroform and ether and trypsin,in acid alkali tolerance assay, the virus was sensitive to Acid and resistant to Alkali,The virus was inactivited by heat to 54 ℃ for 60 minute.Successfully isolated a BVDV strains in Xinjiang and grasp the biological characteristics of the strain. Laid the foundation for future diagnosis and prevention.