Monitoring Of Blood Biochemical Indicators Related To Hypoglycemia, Hyperketonemia, Subclinical Hypocalcemia In Peripartum Dairy Cows

This study monitored the blood biochemical parameters in transitioncows,understood perinatal blood biochemical parameters variation, investigatedincident rate of hypoglycemia, high hyperketonemia and subclinical hypocalcemiacows,and evaluated the diagnostic utility of blood biochemical indicators of the threediseases with retrospective study and preventive medicine opinions.It is designed toprovide experimental evidence for the prediction and early diagnosis of large-scaledairy farm communities hypoglycemia, ketosis, subclinical hypocalcemia and otherproduction diseases.According to expected date of delivery, this study selected thirty, age (2-6yearsold), parity number (2-4) of perinatal healthy Holstein cows to continuous monitor thechanges of blood biochemical indicators. In addition, One hundred and seventy one,age (2-6years old), parity number (2-4) of the perinatal healthy Holstein cows wereselected, and were divided to hypoglycemia group(53), hyperketonemia group(10),subclinical hypocalcemia group(50) and healthy cow group by the measurement ofblood biochemical indicators. In order to evaluate diagnostic effect of bloodbiochemical indicators on the above three diseases, carried out correlation analysis,ROC diagnostic evaluation and the disease risk factors.The experimental results were as follow: The high incidence of hypoglycemiaand hypocalcemia was observed in perinatal cows; hypoglycemia occurred mainlyfrom two weeks prenatal to three weeks postpartum, reaching a peaks on delivery day.The chief indicator for hypoglycemia diagnosis is the serum concentration of glucose,reference indicator includes NEFA, BHBA, TG and AST, their cut-point values are: NEFA(0.44mmol/L),BHBA(0.34mmol/L),TG(0.19mmol/L),AST(53IU/L);Hyperketonemia occurred at1-3weeks postpartum, the highest incidence was at14days-postpartum(10%). The chief indicator for hyperketonemia was BHBA, NEFA andGLU, reference indicators include DBIL, AST, LDH and GGT, their cut-points areDBIL(1.15L), AST(73.50IU/L). Risk factors for hyperketonemia are NEFA(OR=7.72),TG(OR=7.03), GGT (OR=6.708), LDH(OR=4.45) and P(OR=6.23). Hypocalcemiaoccurred perinatal. The chief indicator for hypocalcemia is Ca,The reference indicatoris P. The cut-point for diseases is P (1.99mmol/L), risk factor is NEFA (OR=3.83),GGT (6.71), LDH and (3.84) and Mg (9.57).It is concluded that perinatal cows generally developed physiological negativeenergy balance and negative calcium balance. Hypoglycemia, hyperketonemia andhypocalcemia mainly occurred perinatal period. The chief indicators are GLU forhypoglycemia; NEFA, BHBA, GLU for hyperketonemia and Ca for hypocalcemia.The reference indicators are NEFA, BHBA, TG, AST for hypoglucemia and P forhypocalcemia. First three weeks postpartum is the monitoring time point ofhyperketonemia.