SNPs Detection And Association Analysis With Production Traits Of The Porcine Sirt2、PPARδ、Suv39h1/2Gene

Combined with traditional breeding methods, marker assisted selection (MAS) is widely used inthe process of animal breeding and genetics. This approach accelerates genetic improvement, andmakes an increasingly important role in the breeding work of swine. The basis of MAS is to find outthe major genes which are related with production or reproduction traits in pigs. In this study, weselected Sirt2(NAD＋-dependent deacetylase sirtuin-2), PPARδ (Peroxisome proliferator activatedreceptors δ), and Suv39h1/2(suppressor of variegation3-9homolog1/2) in swine. We extractedgenomic DNA from hair follicles of pigs. As results,537copies of genomic DNA from Landrace, and50from Yorkshire were obtained. With the addition of36copies of Duroc,24copies of Shengxianand50copies of Jiaxing pig, we amplified the target genes. SNPs were obtained by sequencing;genotype frequencies and Hardy-Weinberg equilibrium were analyzed. Particularly, correlationanalysis was taken between SNPs and production traits in586Landrace.The results obtained are as follows:1The product of Sirt2gene is a conservative NAD+-dependent deacetylase sirtuin. Four SNPswere obtained: A59C, A303G, C350G and A488G. We defined three genotypes at each SNP, namedAA, AB, BB, CC, CD, DD, EE, EF, FF, GG, GH and HH. With Chi-square test, two loci (A59C andA303G) were in Hardy-Weinberg equilibrium, and C350G/A488G were significant deviation fromequilibrium. We obtained some results in the correlation analysis between genotypes and productiontraits in Landrace. AA genotype was significantly higher than AB and BB genotype (P <0.05) in birthweight; DD genotype was significantly higher than genotype CD (P <0.01) and the CC genotype (P<0.05) in the average daily gain.2The product of PPARδ gene (peroxisome proliferator-activated receptor) is a member of thesuperfamily of nuclear hormone receptor. One SNP (69586A/G) was obtained and three genotypes (II,IJ and JJ) were named. Chi-square test showed that this locus significantly deviated fromHardy-Weinberg equilibrium. And JJ genotype was significantly better than II, IJ genotype (P <0.05)in backfat thickness.3The product of Suv39h1gene is a member of histone methyltransferase enzyme family. We got two SNPs (655A/G and920C/T), and named six genotypes (KK, KL, LL, MM, MN and NN).Chi-square test showed that the two points are both significant deviation from Hardy-Weinbergequilibrium. Correlation analysis showed there was no significant difference among KK, KL and LLgenotype; and MM genotype was significantly lower than MN and NN genotype (P <0.05) in age of100kg.4Suv39h2is a member of histone methylation gene transfer family. Only one SNPs23301C/Gwas obtained, and three genotypes were named (OO, OP and PP). Chi-square test showed that thislocus was significantly deviated from Hardy-Weinberg equilibrium; correlation analysis showed OPgenotype was significantly better than OO, PP genotype (P <0.05) in weaning weight.