To Separation Of Lactic Acid Bacteria And Yeast Cultures For The Application Of Cow And Newborn Calf Perinatal Care

For screening and research and development for perinatal cows and newborn calvesanti-stress, immune probiotics, in this study were isolated from healthy bovine rumen screenedtwo strains of lactic acid bacteria with antimicrobial activity and resistance as strains. Afterexpanding culture into lactic acid bacteria preparation, and commercialization of viable yeastcultures were mixed to form lactic acid bacteria-probiotics yeast culture preparation for thetransition cows and newborn calves by observing their milk production, calf cattle health, weightgain and detection of blood calcium, inorganic phosphorus and cytokines. Discussion of lacticacid bacteria-probiotics yeast culture application effect, the results were as follows:1Isolates can grow fast to grow well at room temperature between40℃and betweenanaerobic, has good resistance to most drugs are not sensitive to resistance rates of80%, and canat pH3.0and0.3%bovine bile grow well when, with good antimicrobial activity againstEscherichia coli and Staphylococcus aureus inhibition zones were18.3mm and16.4mm.2Number of high-density growth medium with homemade malt juice, solid and liquid mediawere prepared, using MRS solid medium were cultured37.8yuan per liter,24h enrichment was3.2x108CFU/mL, malt share the surrounding market, made of wort, malt juice taken500ml,water500ml, tryptone10g, yeast extract7.5g, Malt juice3g solid medium made of calciumcarbonate added2.38yuan per liter of bacterial culture,24h enrichment to3.5x108CFU/mL, theexperimental results show that the large solid medium wort below MRS solid medium at18.8times the cost in the cell concentration can reach1.1times the MRS medium.3The bacteria of lactic acid bacteria and yeast cultures homemade cows in perinatal care, theexperimentally measured experimental group childbirth24h average milk yield of16.55kg, birthcontrol24h average milk yield of15.42kg;. Experimental high in the control group,1.13kg, weekaverage milk yield higher2kg; prenatal7days hypocalcemia incidence of45%, while80%ofproduction, post-natal declined. Therefore, in this study, lactic acid bacteria and yeast added EMcultures, the control decreased serum calcium. Measured in the control group in the event of theweek prenatal hypocalcemia was31%, reaching a maximum of46.1%of antenatal; experimentalgroup prenatal low incidence rate of16.6%calcium, there are significant differences between thecontrol group. CD4+, CD8+, IFN-γIL-1, IL-2and IL-6were involved in the immune response,activation of macrophages, and improve defenses against viruses and parasites.4Against lactic acid bacteria and yeast cultures homemade newborn calf care in the first10daysin the control group and the experimental group was born calves and two calves weighing less,weight gain is not obvious, because the time period is feeding colostrum and transition milk,produce stress on the calf which affects weight gain. From10days to15days, the rate of weightgain of calves began to accelerate at the end of feeding, although the two calves weight did notshow significant differences, but overall growth to the test group than the control group, theaverage weight is higher than control group2.0kg. Test group and the control group were diarrheaoccurred, but not the same degree of diarrhea in1-10days in the experimental group and thecontrol group there is a greater difference in diarrhea,6.0%higher than the experimental group,the difference was significant. CD4+, CD8+, IFN-γIL-1, IL-2and IL-6were involved in theimmune response, activation of macrophages, and improve defenses against viruses and parasites.