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Rhizosphere Promoting Bacteria Screening And Drought Stress On Tomato Growth

Posted on:2014-10-13Degree:MasterType:Thesis
Country:ChinaCandidate:D L HuaFull Text:PDF
GTID:2283330467957215Subject:Biochemistry and Molecular Biology
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This article through on Xinjiang traditional featured plant resina ferulaerhizosphere microbial and to processing tomato87-5for research material, usedtraditional of microbial filter and the rich set directed single-minded sexual trainingand modern molecular biology means, isolation and screening a strains has ACC(1-amino ring propane-1-carboxylic acid) de ammonia enzyme activity of strains A25,through strains A, on processing tomato seed for processing, and uses PEG-6000simulation drought conditions, on processing tomato drought-tolerant sexual of effect,research indicates that:1the study of isolated from the plant endemic to Xinjiang resina ferulaescreening a strain of bacteria strains with ACC deaminase activity A25, throughmorphology and16SrDNA identified as Sphingobacterium.2on processing tomato seed for processing, its germination and the seed starchenzyme vitality through this research concluded that bacteria liquid A25processingtomato seed in OD600nm Department sucking light value0.5, and soaking4h,germination potential best, germination potential for60%, seed Alpha-starch enzymevitality value is4.4547mg/(g·5min); by PGPR bacteria liquid processing Xinjiangtomato seed,, seed starch enzyme activity as time and concentration of increasedappears peak, decreasing. By one-way anova analysis, root length, projarea, surfareaavgdiam and root volume, significant difference between the groups. Descriptionbacteria liquid A25processing, under different PEG concentration stress tomato rootslength, and projarea, and root surfarea, and root avgdiame; roots volume existsdifferences significantly; after LSD multiple comparison results, roots length, andprojection area, and root surface area, and roots volume in the, bacteria liquidconcentration in OD600nm Department sucking light value0.5, is not with PEG stressprocessing of and bacteria liquid concentration in OD600nm Department undersucking light value0.5, with5%and10%PEG stress differences significantly,Bacteria concentration in OD600nm absorption value at0.5, under the stress of5%and10%PEG, respectively, differences are not significant; the average differences arenot significant.3in drought stress processing, processing tomato within source hormone ABAthrough Duncan multiple comparison,,PEG in concentration for0, and5%in5%level differences does not significantly,10%and0, and5%in5%level differencessignificantly, bacteria liquid concentration, in5%level differences does notsignificantly; drought stress processing tomato within source effects effect acetic acid(IAA),PEG-6000concentration0, and5%, and10%On the0.05level of significanceof difference, solution absorbance value at the0.05level.4the use of PEG-6000under simulated drought on processing tomato seedlingsexposed to different bacteria concentration of catalase, superoxide dismutase,peroxidase and controlled on the0.05level of significance of difference. A25-promoting bacteria in the rhizosphere can delay processing tomato Tomatoestomato resistance to adversity aging or increased capacity.
Keywords/Search Tags:processing tomato, drought stress, ACC deaminase, hormone
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