Analysis Of Transgenic Arabidopsis RNA Interference Based On The Resistance To Aphid

Cotton is an important pest of Xinjiang cotton yield loss caused by the loss ofyield of cotton in Xinjiang each year, causing up to10%~15%, and even up to20%~60%. In addition to cause severe loss of yield, Aphis gossypii Glover also lead tohigh sugar content of cotton fiber, effects of spinning performance. Although theresistance to aphids in enhancement, prevention of Xinjiang cotton aphid still rely onchemical insecticides. Cultivation of aphid species has become an important aim ofcotton breeding. There is no ideal control aphid strategy. RNA silencing is due totransgene sequences or invading virus and was silent endogenous or exogenous genesequence is highly homologous transcripts, the formation of double-stranded RNAstructure, dsRNA can pass through the phloem, reach the target tissue, causingdegradation of specific RNA was recently discovered, the study found interferenceRNA (dsRNA, siRNA) expression can suppress nematode, through the digestive tractof Coleoptera insect and animal genes. On the basis of this principle, by transgenicmethod, expression of dsRNA gene in cotton plants, in order to endogenous genespecific inhibition of feeding cotton aphid, so as to establish a new strategy againstaphids.This study will have the support of three plant built interfering expression ofAphis gossypii by floral dip method into Arabidopsis thaliana, analysis oftranscription levels were selected after feeding on homozygous, then thecorresponding transgenic Arabidopsis V-ATPase-A gene in aphids, Cathepin B,Ribosomal protein L18expression analysis. The results show that these three aphidvectors on the expression of dsRNA in Arabidopsis can succeed, aphids, V-ATPase-Agene and V-ATPase-A gene of aphid interference on the3line transgenic Arabidopsisexpression level was0.17times, feeding on wild type Arabidopsis0.01times and0.04times, in gene were obviously inhibited, successful interference in aphids V-ATPase-Agene, statistical data analysis but inoculated by aphids, not significant on aphidresistance. Aphid body protein L18gene in transgenic Arabidopsis Ribosomal3linein the aphid interference gene Ribosomal on the expression of protein L18was0.27times, feeding on wild type Arabidopsis0.28times and0.27times, the gene wassignificantly inhibited, the successful interference aphids in vivo gene V-ATPase-A,statistical analysis of data but not inoculated by aphids, notable for aphid resistance.Although the study on the application effect is not obvious, perhaps due toman-made aphid damage, significant inhibition of gene expression analysis but, thisstudy provides a theoretical basis for the new strategy of aphid resistance breeding. At the same time, this method of transgenic plants to provide new ideas for gene functionanalysis of aphids.