The Design Of Ivermectin-SiO₂Slow-release Nanogranuce And The Establishment Of Method For Measuring Ivermectin In Sheep Palsma

Parasitic disease is one of the important factors which harm the healthydevelopment of agriculture industry in China. As a broad-spectrum, high efficiency, lowtoxicity antiparasitic medicine, ivermectin is widely used by people. Ivermectin traditionalformulation needs repeated administration for the purpose of treatment, which can increaselabor costs and cause resistance easily. The research of a new drug original powder is inputmuch money and taken a very long time, and the effect of the medicine is not only related tothe medicine itself, but also has an important link with the pharmaceutical dosage form.With the development of science and technology, people can use some nanomaterials as thecarrier to prepare new nano-pharmaceutic preparation, especially the nano-silica particles.Inorganic nano-silica composite material is non-toxic and has a good biocompatibility,which is an ideal choice as medicine carrier. It can stay a long time in the mucosa, cornea,etc of the body because of the small particle size and high surface energy, so we can increasethe medicine residence time in the body and improve the biocompatibility of the insolublemedicine.1. Purpose: The purpose of this study was to design a formulation using silicone ascarrier, getting the nanoparticles of having different drug loading in aqueous solution, so thatrelease of ivermectin (IVM) can be controlled for a long period of time. Methods: It wasknown that whether the drug was loaded by using Fourier transform infrared (FT-IR). Theparticle shape and particle size of drug-loaded particles was observed by transmission electronmicroscopy (TEM). The actual loading weight percentage of silica nanoparticles loaded withdrugs was analyzed by using thermal gravimetric analysis and high performance liquidchromatography (HPLC). The release rate was researched by release dialysis test. Results:The drugs had been successfully adsorbed by silica nanoparticles without degradation andthe loading weight percentage could be controlled. The drug-loaded particles had goodspherical morphology by TEM observation; their minimum particle diameter was30nm andthe largest was50nm; the average particle diameter was about40nm; the particle wasagglomeration. There was little difference about the actual drug loading and theoretical drug loading. Cumulative sustained release rate was more increasing with the increase in theloading weight percentage, but all less than IVM release rate. The silica nanoparticles whoseloading weight percentage was big, had more evident sustained release phenomenon after90h. Conclusion: In contrast to a fast and short-term release from pure drugs themselves, thedrug loaded silica nanoparticles showed a slower and longer release. This work provided anew way to IVM loaded silica nanoparticles for the controlled release. Simple preparationand low cost made it possible to be used in clinical practice.2. Purpose: Establishing the method of measuring ivermectin in the sheep plasma.Methods: We might determine the ivermectin content of the plasma by high performanceliquid chromatography (HPLC). The detection conditions: The column was Agilent TC-C18(5μm，4.6x150mm，I.D.)；the mobile phase: methanol:water＝0.5:0.05(v/v); columntemperature30℃; detection wavelength254nm；the injection volume20μL. Results: Thelinear correlation coefficients of the standard curve between the medicine-blood samplesolution and standard solution was0.9990and0.9994, linear range was0.025-5μg/mL, thelinear relationship met the requirements, percent recovery was93.4%, it was ok, the lowestdetectable limit was0.005μg/mL. Conclusion: The method of extraction and purification ofblood was simple, the results was credible by detecting with high liquid chromatography.