Effect Of JMJD1C On Cumulus Cell Proliferation And Apoptosis Of Cattle

Cumulus cells, as a kind of granulosa cells, its expanded states and apoptosisratios impact oocytes maturation and embryonic development. BMP15, IGF-I andFSH can promote granulosa cell proliferation, growth and differentiation, contributingto the normal maturation of oocytes. JMJD1C is a member of KDM3families.JMJD1A and JMJD1B (KDM3A, KDM3B) can remove H3K9me1and H3K9me2methylation modification specifically, However whether JMJD1C also has a H3K9catalytic action is unclear and its effect on cumulus cell apoptosis and proliferationmediated by BMP15, IGF-I and FSH is still rarely reported.In order to clarify the role of JMJD1C’s action of H3K9demethylation. Afterbovine cumulus cells cultured in viro, we used RNAi methods inhibit JMJD1C mRNAexpression. Immunofluorescence results shows that H3K9me1and H3K9me2expression level are no significant changes after JMJD1C mRNA expression in bovinecumulus cells is suppressed successfully. That is to say JMJD1C has unapparent effecton the removal H3K9me1and H3K9me2methylation.Different concentrations of BMP15, IGF-I, FSH are added in the culture mediumseparately and detecting the impact on JMJD1C expression level mediated by BMP15,IGF-I and FSH. Real-time PCR results show that with the increasing of FSH andIGF-1concentrations, there is no significant difference in the JMJD1C mRNAexpression; With the increasing of BMP15concentrations, there is significantdifference in the JMJD1C mRNA expression when dosage is50ng/ml (p <0.05).Using MTS cell proliferation method, the results show BMP15can promote thecumulus cell proliferation significantly when its dosage is50ng/ml. Experimentalresults show that the addition concentration BMP15was50ng/ml, significantlyinhibited the expression of JMJD1C and promote the cumulus cell proliferation.Therefore, we further selected50ng/ml dosage in order to explore JMJD1C’sinfluence on cumulus cell apoptosis and proliferation mediated by BMP15.JMJD1C gene is silenced in primary culture of Bovine cumulus cells. Detecting-genes related in between mRNA level and protein level expression, Bcl2and Bax ratios are significantly higher. Cumulus cell apoptosis ratios decrease afterBMP15is added and JMJD1C is silenced at the same time. The results show thatcontact between BMP15and JMJD1C can effect cumulus cell apoptosis, JMJD1C caninhibit cumulus cells anti-apoptotic mediated by BMP15. The results show that theindirect links between BMP15and JMJD1C can effect cumulus cell apoptosis,JMJD1C can inhibit cumulus cells anti-apoptotic effects mediated by BMP15.In summary, this study clarify at the mRNA level JMJD1C do not directlyregulate H3K9me1, H3K9me2demethylation in cumulus cells, JMJD1C can inhibitcumulus cells anti-apoptotic effects mediated by BMP15. The results will help toidentify differences among of KDM3related proteins closely. The function ofJMJD1C is explored originally and provide of the molecular mechanism of cumuluscells apoptosis.