Oxidative Stress On Boar Reproductive System Damage And Intervention Effect Of Acidic Amino Acids

The experiment was conducted to establish the oxidative damage model by means of intraperitoneal injection of50ml10%hydrogen peroxide (H2O2) and and scrotal injection of20ml10%H2O2(referred to "abdominal injection" and "outside injection").The effects of oxidative stress (OS) on mature boar testis morphology.organ coefficient of testise and pididymis,relative expression of mRNA in inflammatory mediators of tissues TNF-α、IL-6、 IL-10、IL-1β、TGF-β1).hormone content of FSH、LH、T in serum, effects on antioxidant capacity were investigated for the first time. And the effects of2%glutamate (Glu)and2%aspartic acid (Asp)added into dietary on the indicators above were discused in this study.The36average weight for85kg sexual maturity in large white boars June age of, were randomly divided into9treatment,Control group A、The test group B (sampling,1days after abdominal injection)、The test group C (sampling abdominal injection and fed the experimental diets,7days)、The test group D (abdominal injection and were fed the experimental diet+2%Glu�'7days after sampling)、The test group E (abdominal injection and were fed the experimental diet+2%Asp�'7days after sampling)、The test group H (sample injection,1days)、The test group I (sample injection, were fed the experimental diets,7days)、The test group J (external injection and were fed the experimental diet+2%Glu�'7days after sampling)、The test group K (external injection and were fed the experimental diet+2%Asp�'7days after sampling).The results are as follows:1.Testicular biopsy shows:Mature boar OS after its testis tissue had obvious pathological changes.Compared with the control group, the test group had different degree of seminiferous tube degradation;Spermatogonia, spermatocytes and spermatids in the seminiferous tubules arranged in disorder, cell atrophy.Adding2%Glu,2%Asp failed to repair damage to testicular tissue.2.The results showed that the organ coefficient:Abdominal and scrotal injection of H2O2will increase the mature boar testis coefficient.But the testicle coefficient B of the experimental group and the experimental group H was significantly lower than C group and I group(P<0.05),Test the vulva capsule injection of H2O2group, the testis coefficient than the test group were injected H2O2of high.Test of intraperitoneal injection of H2O2group had no significant change in the epididymis coefflcient(P>0.05).Test and injection of scrotum H2O2group significantly increased the epididymis coefficient(P<0.05).Experiments of adding2%Glu,2%Asp group, the boar testis coefficient has a trend of decline in the abdominal and scrotal after injection of H2O2,But the group of external injection of scrotum H2O2decreased more significantly(P<0.05)But between these two kinds of AA had no significant difference (P>0.05).And no influence of epididymis coefficient of test group.3.The determination of FSH, LH and T in serum shows:Abdominal and scrotal injection of H2O2can make sexual mature boar serum FSH, LH and T change. The test group B and group H was slightly lower than the experimental group C and group I.The test group were injected H2O2of group A LH. T were significantly different compared with control A(P<0.05),Vulva capsule injection of H2O2in experimental group than in the control group had significant difference between A and FSH (P>0.05).Adding2%Glu,2%Asp after FSH, OS did not improve the boar LH and T in serum.4.The results showed that GSH-Px、SOD activity and MDA content in serum:Abdominal and scrotal injection of H2O2can make sexual mature boar serum MDA increased significantly (P<0.05),SOD activity decreased,At the same time feedback caused by compensatory enhancement of GSH-Px activity,But the differences were not significant (P>0.05).Adding2%Glu,2%Asp for the boar in serum after intraperitoneal injection of H2O2MDA decreased significantly (P<0.05),Difference between the experimental group and the addition of2%Asp more significantly (P<0.05),But the serum on boar and scrotal H2O2after injection of SOD, GSH-PX activity and MDA content had no significant effect (P>0.05).5.The testis and epididymis related inflammatory factor mRNA relative expression quantity display results:Expression of TGF-β1testis weight after intraperitoneal injection of H2O2of the test group decreased (P<0.05),But in test group scrotal injection of H2O2increased (P>0.05),The expression of TNF-β, TGF-β1expression and the opposite;Expression of IL-1β were significantly increased in the experimental group were intraperitoneal and scrotal injection of H2O2(P<0.05);IL-6, IL-10expression levels of performance for the test group and the test group B、H、A lower than the control group,And the test group C and group I was higher than the control group A, and with the test time increases the expression decreased first and then increased.And the epididymis gene expression after intraperitoneal injection of H2O2showed a downward trend,But there are still significant difference with the control group A (P<0.05);Expression of vulva capsule injection test H2O2group the TNF-alpha continued to decline,Expression of TGF-1β1and the test group H, IL-10, IL-6were significantly lower than I group (P<0.05),The test group H, I and the expression of A in the control group had significant difference (P<0.05).Adding2%Glu,2%Asp on the expression of TNF-a in the testicular tissue in abdominal and scrotal injection of H2O2has a little influence on the experimental group (P>0.05);Expression of IL-1β were obviously decreased (P<0.05), the test group injection of scrotum H2O2decreased more significantly (P<0.05);The test group the expression of IL-6, TGF-(31injection in abdominal cavity of H2O2is on the rise.The expression of IL-10in the intraperitoneal injection of H2O2added2%Asp experimental group significantly decreased (P<0.05),And out of the scrotum H2O2injection test group did not change significantly;The expression of OS gene in the boar epididymis adding2%Glu,2%Asp in the diets were rising trend,And the test group scrotal injection of H2O2increased more significantly (P<0.05).The test results show:Abdominal and scrotal injection of H2O2will cause a mature boar testis injury group than in the control. Combined, organ coefficient,Increased FSH, LH, T content decreased, MDA content in serum,The expression of inflammatory factors increased,And the injection of scrotum H2O2changing the way is more obvious.And the effect of adding2%Glu,2%Asp diet does not make the testicular tissue injury recovery,But the testicle coefficient has a downward trend in the two injection mode,The test group vulva capsule injection of H2O2decreased significantly (P<0.05),But between these two kinds of AA had no significant difference (P>0.05).Can not raise serum FSH, LH, T content;MDA can reduce the serum levels by intraperitoneal injection of H2O2,Difference between the experimental group and the addition of2%Asp more significantly (P<0.05),But the serum on boar and scrotal H2O2after injection of SOD, GSH-Px activity and MDA content had no significant effect (P>0.05).Diet can be supplemented with2%Glu,2%Asp decreased to a certain degree of injection H2O2OS expression amount of boar orchitis factor,But between these two effects of amino acids on the gene is not big difference.In addition, the test results show,All treatments were not restored to pre stress levels,The exterior and test performance during the same breeding.All tests to both groups sampled,The outer scrotal than the control group still showed different degrees of swelling phenomenon.