Subchronic Toxicity Of Sulfur Additive On Rats

This experiment is to investigation the subchronic toxicity of sulfur anion feed additive on rats and effects of liver antioxidant capacity. Select 80 SD rats, male and female. Were randomly divided into four groups, Each group of 10 male and a female, The control group were fed with normal diet; low-dose group, fed diets containing 0.3%　sulfur anion feed additives feed; dose group, fed diets containing 0.9%　sulfur anion feed additives feed; high-dose group, fed diets containing 2.7%　from Phoenix anion feed additives feed.　90 days of continuous feeding. At day 45 to pick six rats in each group, all three male and female, were blood, autopsy, organ weighed. At day 90, all rats were blood, autopsy, organ weighing, measuring and grinding liver MDA, GSH, SOD specific activity, observe kidney of rats in high dose group with histological observation.Test Results:(1). Changes in body weight of rats:　the basic situation during the test animals in each group is good, no anorexia, death and other abnormal behavior, body weight in rats by recording weekly, found that female high-dose group　at day 28 and day 49 and-the control there was significant difference (P<0.05), the rest of the group and the control group showed no significant difference.(2). Feed efficiency: Early feeding sulfur anion feed additives, feed utilization experimental group was significantly lower than the control group, as the trial, feed efficiency has been improving. The feed efficiency to the sixth week of the start of the test group was higher.(3). Organ coefficient: The organ coefficient in mid-term test and high dose groups of female rats lungs was higher and significantly different (p<0.05), the rest of the group and the control group showed no significant difference; End of the experiment, Low-dose group and kidney organ coefficient compared to the control group of male rats significantly different (p<0.05), Dose group-female rat thymus organ coefficient compared with the control group, a significant difference (p<0.05), the rest of the group and the control group showed no significant difference.(4). Hematology test results: Sulfur anion 90d feeding study, mid and late, male and female rats, low, medium and high dose groups of hematology test results compared with the control group showed no significant difference.(5). Serum biochemical examination results: Mid-term test Female low dose group and high dose group was significantly higher AST, and the control group, the difference was significant (p<0.01), High alkaline phosphatase levels in male high dose group compared with the control group, there was a significant difference (p <0.05); End of the experiment, There was no significant difference compared to male and female rats of each group serology test results with the control group.(6). Histological findings:By optical microscopy on tissue sections and found no abnormal tissue.(7). Liver antioxidant capacity:　No significant impact on the content of rat liver MDA and glutathione, no significant difference (p<0.05) with the control group; Liver superoxide dismutase (SOD) specific activity also have a significant effect, test group SOD specific activity higher than the control group, dose group statistically significant, (p＜0.05).The experimental results show sulfur anion feed additives fed rats 90 days did not produce toxicity, and can enhance the antioxidant capacity of rat liver. In this experiment, the optimal concentration of 0.9%.