Correlation Research On Ploidy Breeding Of Plants Of Hemerocallis

30 plants of Hemerocallis as the test material identified their ploidy and the relationship between chromosome ploidy and stomata traits determined in this thesis. This paper studies period of microspore and bud exterior form corresponding relation of the Hemerocallis materials. Hemerocallis materials are studied belong to the genotype of materials, types of culture medium, sucrose concentration, ratio of hormone on the in vitro culture and callus induction of anther. Genotypes were studied and sucrose concentration on the influence of culture in vitro of unfertilized ovary; The research of the variety of hormone and grouting effect on ovary into seedlings. Major research results:1.The Ploidies of collected 30 materials of Hemerocallis were determined, And to explore the relationship between times and design and color. The results show that:the 23 material:chazi variety, qiezi variety,panlong variety,liuyu variety,datong variety,xiaohuang variety, beihuanghua variety, wild datong variety,qiaotou variety,guanglinghua variety,yaozitou variety,shijing variety, xiaoyu variety, changzuizi variety, siyuehua variety, chonglihua variety, dalihua variety, baihua variety, gansu xianhuanghua variety, xiaohua huanghua variety, Malian huanghua variety, huohuanghua variety, gansu gaoting variety are diploid,2n=2x=22,and the flower colors are yellow, the 7 material:malin variety, chongqinghua variety, gaoting variety, xianhuanghua variety, huaian huanghua variety, duanbang heizui variety, wild lishan huanghua variety are triploid,2n=3x=33,and the flower colors are red.2.Statistics of 30 materials of Hemerocallis of material stomatal traits, And determine its relationship with the ploidy. The results show that:Stomatal density and stomatal guard cells chloroplast number can be used as a preliminary judge index of ploidy identification..3. The period of microspore of materials of Hemerocallis with bud external form. The results show that:Mononuclear period corresponds to the side of microspore bud length is 10～12 mm, and the Anther for the green. Middle mononuclear corresponding bud length is 9～10 mm, and the anther for white and green. So the anther culture bud based range for 9～12mm.4. Selecting genotype, medium type, sucrose concentration and the ratio of hormones of callus induction of anther in vitro culture on materials of Hemerocallis. dalihua variety is the materials of Hemerocallis in anther culture of callus induction of optimal material. Anther culture the optimal culture medium for callus induction of increase the MS culture medium of vitamin B1 to 4mg/L medium. Anther culture of callus induced the optimal sucrose concentration was 6%. The optimal hormone anther culture of callus induction ratio was NAA0.5mg/L+KT 0.5mg/L.5. Selecting the best genotype and sucrose concentration of the materials of Hemerocallis culture in vitro of unfertilized ovary, and getting regeneration plant. Dalihua variety is the materials of Hemerocallis culture in vitro of unfertilized ovary of optimal material. materials of Hemerocallis culture in vitro of unfertilized ovary of the optimal sucrose concentration was 6%. Materials of Hemerocallis induced shoots the optimal medium for MS culture medium+3% sucrose+NAA 0.5mg/L+KT 2mg/L.Materials of Hemerocallis induced by rooting the optimal culture medium for MS culture medium+3%sucrose+NAA 2mg/L+6-BA 0.5mg/L.6. Getting regeneration plant and a plant haploid plants in it in this test by identificating the ploidy of materials of Hemerocallis culture in vitro of unfertilized ovary of regenerative plants.