| Pardosa astrigera are poikilothermic animals, the heat that their life activities required comes mainly from the environment. In recent years, greenhouse effect the vagaries of weather, pest ravaged farmLand. As very effective biological predators for agricuLtural pest control, exploration on the influence of temperature for pardosa astrigera can not only make better use of pardosa astrigera, reduce the pesticide application of farmLand, but also provide a reference for spider indoor farming and some theoretical basis for forecasting the occurrence of pardosa astrigera. In this paper, as the natural enemies of common insects on Yangtze River, Yellow River Basin farmLand, pardosa astrigera are studied. From-2℃ to 40℃, the impact of behavioral activity, physiological and biochemical indexes, resistant gene expression on Pardosa astrigera were investigated. The resuLts are as follows:1. After the temperature treatment, Pardosa astrigera capacities were recorded by small animal behavior Locus Analyzer Ethovision. The total distance, average speed and great walking speed of Pardosa astrigera gradually increased as the temperature increases from -2℃ to 16℃, decreased as the temperature rises after 16℃, and the walking ability of Pardosa astrigera also decreased. The number of passed through regions in the test chamber was significantly reduced at -2℃,40℃. After treatment at other temperature, the difference was not significant. the males have a strong behavioral activity at low temperatures.2. The moisture, fat, trehalose and glycerol four indicators were selected, the physiological and biochemical changes of Pardosa astrigera affected by temperature were determined.1) The free water content of Pardosa astrigera was investigated by means of physical drying. It decreased as the temperature rises from -2℃, and reached the minimum value at 24℃. When the temperature was higher than 24℃, the body water content of Pardosa astrigera increased as the temperature rise. The males had more water loss at low temperatures, and had a strong resistance capability to low temperature.2) The body fat content of Pardosa astrigera was investigated according to the similarity principle compatibility. It decreased in the process of temperature increasing from -2℃ to 12℃, and increased as the temperature rise from 24℃ to 40℃. The body fat content of males showed large variations.3) The body trehalose content of Pardosa astrigera was investigated using the anthrone method. It presented a "V" in the temperature range from -2℃to 40℃, and reached the minimum value at 22℃. The body trehalose content of females was greatly affected by temperature, it was higher than that of males under the same conditions of temperature. The females had a strong resistance capability to temperature changes.4) The body glycerol content of Pardosa astrigera was also investigated according to periodic acid oxidation-reduction method. It decreased as the temperature rises from -2℃. Low temperature prompted Pardosa astrigera reserving more glycerol.3. After treatment at different temperatures, the HSP70,90 gene fragment expressions of Pardosa astrigera was detected using fluorescence quantitative PCR method. HSP70,90 basically did not expression at the temperature range of 16-26℃. The expressions of HSP70,90 increased when the temperature dropped from 16℃ to -2℃. And the expressions of HSP70, HSP 90 were 334.53 times and 9.38 times of the control group respectively. The expressions of HSP70,90 increased as the temperature rises from 26 ℃, and reached the maximum value at 38℃, the expressions were 716.46 times and 10.44 times of the control group respectively. As the temperature continues to rise, HSP70,90 gene expressions were inhibited and decreased at 40℃ After 2 hours temperature treatment for Pardosa astrigera, HSP70 can be fast expressed, but the expression of HSP90 is lagging behind. |
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