Imported Wheat Pest Risk Analysis And Two Kinds Of Wheat Disease Quarantine Detection And Monitoring Technology

On the entry of wheat pest risk analysis. Wheat leaf blight(Alternaria triticina) and wheat India bunt fungus(Tilletia indica) PCR detection methods were studied and two diseases occurred in the port area monitored the situation.We assessed the entry of wheat carried pest,There are 31 kinds of pests and quarantine-related,Wher-e in the nine kinds of insects, six species of Nematode,five species of Fungi,three species of Viruses,eight species of Weed s.Based on the results of the risk analysis,Wheat leaf blight(Alternaria triticina) as research subjects, To study its growth characteristics in different culture conditions,Determine the biological characteristics o-f the pathogen. After the cultivation of wheat leaf blight 7d under different culture conditions and culture e-nvironment measurement colony diameter, With SPSS18.0 software measurement data systems analysis. T-he bacteria optimum growth medium is PDA and PCA;CMA and WA are not suitable for the growth of ba-cteria; The bacteria optimum carbon source glucose, sucrose and starch, The optimum nitrogen source sodi-um nitrate and glycine, In PH4.0-12.0 could grow, In the 24 h light conditions conducive to the growth of mycelium.Homologous alignment analysis after India wheat bunt fungus-specific primers for the test sample and the resulting products were sequenced,Specifically for the test sample and foreign reports fishy Indian strain of wheat was 100% homology. In imports of wheat and wheat taxkorgan PCR testing, were found to carry the bacteria.The wheat leaf blight were cloned and sequenced, The resulting sequence according to the sequencing of the bacteria designed to detect specific primers LJY1 / LJY2, FZYF / FZYR and TaqMan probes FZYX, Optimize obtained after detecting the bacteria optimum ordinary PCR and real-time PCR system,Test r-esults showed that both methods can quickly detect wheat leaf blight.Within two years Research in Tilletia indica Mitra and Alternaria triticina Prasada & Prabhu in five ports in XinJiang. In the conduct of the various ports Spore Capture、Wheat monitoring goods entering the country and Sentinel surveillance in ports, It was not found in Tilletia indica Mitra and Alternaria triticina Prasada & Prabhu. Description of these two pathogens not yet passed five ports in Xinjiang.