Studies On Solid State Fermentation Of Camellia Meal By Combination Strains

The main components of oil-tea meal are introduced briefly in the paper and the problems of how it be used as fertilizer are also presented. There are nearly21.7%cellulose and13.8%tea saponin in the oil-tea meal’s composition. The tea saponin has effects of anti-bacteria in soil to a certain extent, and the degradation of cellulose contained in meal may increases the temperature of soil and then injured the roots of plants. The solid-state fermentation of oil-tea meal by mixed bacteria was conducted in this paper in the realize of faults of meals applied as fertilizer directly.Three cellulose degrading bacteria YY-2, YY-3and YY-6were screened from soil, and the best combination of mixed strains D8+YY-3was decided after a series of antagonistic experiments with three saponin degrading strains conserved in the lab. The degradation rates of cellulose and saponin are39.61%and50.49%respectively by the mixed strains. Optimization of liquid fermentation and1kg-level solid-state fermentation were conducted successively. On the base of these optimal conditions, expanded and validation experiments were conducted soon afterwards, and the results are as follows:1. the optimal fermentation conditions are temperature37℃, inoculation12%, fermentation time48h, shaking speed175r/min, initial pH8, and the best inorganic ion is calcium ions in the liquid fermentation by the mixed strains D8+YY-3. orthogonal experiments were then conducted and the optimal conditions are follows:inoculum12%, calcium ions1%, temperature37℃, tea meal10%, initial pH8. validation experiment shows the results that biomass of fermentation is5.41g/L, and the degradation rates of cellulose and saponin are41.47%and56.31%respectively.2.The results of optimization of solid-state fermentation are follows:the water content of solid packing material is50%, inoculum’s size is16%, and stirring rate is once every three days, and the fermentation period is11days. Under these optimal conditions, the degradation rates of cellulose and saponin are39.09%and54.27%respectively, the water content is23.17%, and pH of degradation products is7.3by the validation experiments.