Effects Of Ultra-fine Tradition Chinese Medicine Compound On Lipid Metabolism And Related Gene Expression In Different Layer Breeds

The experiment was conducted to determine the effects of ultra-fine tradition Chinese medicine compound(TCM) on lipid metabolism and related gene expression of 2 native chicken breeds in Jiangxi and Hyline laying hens. 90 300-day-old Chongren chicken, Yugan blackfeather chicken, and Hyline laying hens about similar weight respectively were randomly assigned to 2 groups. Control group layers were fed with a basal diet, the layers in experimental group were fed with the basal diet containing 1% TCM in the whole trial period of 60 days. Egg cholesterol content, production performance, serum lipid and fat deposition were determined on days 1, 15, 30, 45, and 60; and the expression of apolipoprotein Aâ… (apo Aâ… ) and apolipoprotein B100(apo B100) m RNA in liver, kidney and small intestine were determined on days 1, 30 and 60, by the method of real-time PCR. The results are as follows:1. The experimental group compared with the control group, serum triglyceride(TG), total cholesterol(TC) and low-density lipoprotein cholesterol(LDL-C) levels significantly decreased(P<0.05) in 3 chicken breeds, which serum high-density lipoprotein cholesterol(HDL-C) levels significantly elevated(P<0.05). As time went on, the levels of serum TG appeared upgrade firstly then descending latter, while serum HDL-C levels had a downward trend in the trial time.2. Compared with control group, hepatic fat percentage, liver index, and abdominal fat percentage significantly decreased(P<0.05) in experimental group of 3 laying breeds, which consistent with the change trend of serum lipid levels in this trial. There were no significant difference(P>0.05) in muscle fat percentage among groups.3. Compared with control group, the expression of apo B100 m RNA significantly increased in liver and kidney in experimental group of 3 laying breeds, the expression of apo Aâ…  m RNA significantly increased(P<0.05) in liver, while there was no significant difference(P>0.05) in kidney; the expression of apo Aâ…  m RNA significantly increased(P<0.05) in small intestine, while there was no significant difference(P>0.05) in the expression of apo B100 m RNA.4. The present study revealed a significant decrease(P<0.05) in egg cholesterol content after dietary supplementation with TCM, and there was no significant difference(P>0.05) in laying performance among groups.In conclusion, dietary supplementation with TCM could be used to reduce egg cholesterol content by improving lipid metabolism, along with the expression of apo Aâ…  and apo B100 m RNA increasing in tissues.