Analysis Of Functional Components Of Different Pecan(Carya Illinoinensis) Clones

29 varieties of pecan [Carya illinoinensis(Wangenh.) K. Koch] were determined and analyzed for their tocopherols, ellagic acid, tryptophan and so on, which revealed the difference in the content of functional components between different pecan clones. Besides, this study provided a theoretical basis for comprehensive evaluation and utilization of pecan clones and laid a good foundation for further promotion of pecan as a dietary supplement. The results were as follows:1. 29 pecan clones were determined and analyzed for their total polyphenols(TPs), total flavonoids(TFs) and antioxidant capacity(AC). The Folin-reagent method was used to determine TPs, the sodium nitrite-aluminium nitrite method was used to determine TFs, at the same time, both ABTS assay and DPPH assay were used to determine AC. As a result, TPs ranged from 10.99~47.96 mg GAE/g and TFs ranged from 3.65~21.50 mg RE/g in the defatted kernels of pecan. ZL86 showed the higher TPs content and AC value. TPs, TFs and AC were significantly different among different pecan clones, respectively. Strong correlations were found in kernels between AC and TPs as well as AC and TFs. Cluster analysis indicated group Ⅱshowed the higher TPs, TFs and AC.2. To optimize conditions for the ultrasonic extraction of ellagic acid from the defatted kernels of pecan. The optimal extraction conditions of ellagic acid were ethanol concentration of 61.99%(V/V), extraction time of 21.83 min, solid to liquid ratio of 1:27.97(g/m L). The content of ellagic acid in the defatted kernels of 29 pecan clones were detemined by using the optimization extraction conditions.The result showed the content of ellagic acid ranged from 60.62 to 222.40 mg/100 g. Ellagic acid were significantly different among different pecan clones. Besides, ZL69 showed the highest ellagic acid content.3. The content of tocopherols in pecan oil were detemined by reversed-phase high performance liquid chromatography(RP-HPLC). As a result, the content of α–tocopherol ranged from 0.41 to 1.22mg/100 g, the content of δ–tocopherol ranged from 0.91 to 3.18mg/100 g and the content of γ–tocopherol ranged from 8.73 to 26.14mg/100 g which showed the highest contents among the tocopherols in pecan oil. The total tocopherol content ranged from 10.03 to 29.82 mg/100 g. This study also showed that there was significant difference in the content of tocopherols of pecan oil from 29 clones. Besides, ZL76 pecan showed the highest content of γ–tocopherol and total tocopherols.4. The content of tryptophan in defatted kernels of pecan were were detemined by RP-HPLC. The result showed the content of tryptophan ranged from 172.86 to 352.22mg/100 g. Tryptophan were significantly different among different pecan clones. Besides, ZL70 pecan showed the highest tryptophan content.5. In conclusion, took the content of TPs, TFs and AC into consideration, ZL86 were selected. Took the content of ellagic acid into consideration, ZL69 were selected. Took the content of tocopherols into consideration, ZL76 were selected. Took the functional components above and former conclusions into consideration, ZL87、ZL84、ZL83、ZL90、ZL85 were selected to develop the health care function of pecan.