Characterization And SNP Polymorphism Study Of LncRNA-pouBW1 In Chicken

Poultry’s growth rate has been the research focus of scientific workers.We found a new gene in the studying of growth speed difference of Gushi chickens and Anka broiler. In order to study the biological characteristics and the function of the gene further, the ORF(open reading frame) of the gene was cloned by connecting with T-vector and quantitative Polymerase Chain Reaction(q PCR) was performed to analyze its expression characteristic in different tissues. Then we caarried out the association analysis in F2 population of Gushi chickens and Anka broilers. We get the results as follows:① The complete ORF of the gene, which is as long as 918 bp, was cloned, designated as pou BW1, and submitted to Gen Bank(accession no. KP147948). The pou BW1 gene was located on chromosome 2 and only found in chicken by bioinformatics analysis.② The results of q PCR showed that the pou BW1 gene was generally expressed in chicken. Highest abundance of pou BW1 was found in the spleen and lung, while lowest abundance in pectoralis and leg muscle.③ We found two SNP site( n.594C>T and n.830G>A) by DNA pool. The polymorphism of the gene was dramaticlly associated with leg muscle weight and the body weight at the age of 1day, 4 weeks and 6 weeks. The chickens with AA genotype were dramaticlly higher than those with GG genotype.We concluded that the gene may play an important role in regulation chicken growth. The lowest relative m RNA level of pou BW1 in pectoralis and leg muscle, a comparison with the results we have studied, we support the hypothesis that the gene promoted the development of chickens’ leg muscle by negative feedback control,which needs to be further confirmed.