| The author got Macrocystis pyrifera young sporophyte on October 8,2013 from researcher Wang Fei jiu in Yellow Sea Fisheries Research Institute.Chinese Academy for the study.She set four light intensity treatment (50,100,150,200μmol/(m2·s)), four temperature treatments (5,10,15,20℃) and four salinity treatment (20,25,30,35), experiments fixed one of the environmental factors, and change the other two environmental factors to culture young sporophyte,after culture experiment.she determined the specific growth rate, protein content, chlorophyll content, material accumulation, sodium alginate content in order to provide a theoretical basis to farm kelp in China or exhibition pavilions.1.light intensity and temperature on Macrocystis pyrifera young sporophyte growth and biochemical compositionThe SGR of Macrocystis pyrifera young sporophytes was influenced by light intensity, temperature,and the interaction between the two factors.When the light intensity was constant,as temperature rose, SGR increased and then decreased. When the temperature was constant, as light intensity rose, SGR increased first and then decreased. Therefore, when the light intensity was 100μmol/(m2·s) and temperature was 15℃, SGR reached the maximum(18.401), when the light intensity was 50μmol/(m2·s) and when the temperature was 5℃, SGR reached the minimum(2.358).The protein contant of Macrocystis pyrifera young sporophytes was influenced by light intensity,temperature,and the interaction between the two factors.When Light intensity was constant, as the temperature rose, the protein content is substantially increased gradually. When the temperature was constant, as the light intensity rose, protein content increased then decreased. Therefore, when temperature was 20℃ and light intensity was 100μmol/(m2·s), protein content reached the maximum(17.766mg/g). when temperature was 5℃ and light intensity was 50μmol/(m2·s). protein content reached the minimum (2.502mg/g).The Chlorophyll a of Macrocystis pyrifera young sporophytes was influenced by light intensity,temperature, and the interaction between the two factors.When light intensity was constant, as temperature rose, the chlorophyll a content was basically a drop after the first rise. When temperature was constant,as light intensity rose, chlorophyll a content essentially decreased, but the temperature of 10℃ group had not such trend.Therefore,when light intensity was 50μmol/(m-·s) and temperature was 15℃, chlorophyll a reached the maximum (1.203mg/g), when light intensity was 150μmol/(m2·s) and temperature was 5℃, chlorophyll a content reached the minimum (0.425 mg/g). The Chlorophyll c of Macrocystis pyrifera young sporophytes was influenced by light intensity, temperature,and the interaction between the two factors.When light intensity was constant, as temperature rose, the content of chlorophyll c basically dropped after the first rise. When temperature was constant, as light intensity rose, the content of chlorophyll c substantially decreased. Therefore, when light intensity was 50μmol/(m2·s) and temperature was 15℃, chlorophyll c content reached the maximum(6.545mg/g), when light intensity was 150μmol/(m2·s) and temperature was 5℃, chlorophyll c reached the minimum(2.076mg/g).The carbon content of Macrocystis pyrifera young sporophytes was never influenced by light intensity, temperature, or the interaction between the two factors.When the temperature was 10’C and light intensity was 150μmol/(m2·s), the carbon content reached the maximum (31.8%), when the temperature was 20℃ and light intensity was 100μmol/(m2·s), carbon content reached the minimum (28.50%). Nitrogen content of Macrocystis pyrifera young sporophytes was never influenced by light intensity, temperature, but they was influenced by the interaction between the two factors.When light intensity was constant, as temperature rose, the nitrogen content was gradually reduced. When temperature was constant,as light intensity rose, the nitrogen content reduced then increased. Therefore,when temperature was 5℃ and light intensity was 50μmol/(m2·s), nitrogen content reached the maximum (4.36%),when temperature 20℃ and light intensity was 150μmol/(m2·s), nitrogen content reached the minimum (3.54%).The sodium alginate content of Macrocystis pyrifera young sporophytes was significantly influenced by temperature,but they was never influenced by light intensity, or the interaction between the two factors.When light intensity was constant, as the temperature rose, sodium alginate content increased and then decreased.When temperature was constant, as light intensity rose, sodium alginate content increased and then decreased, but the difference was not significant. Therefore, when temperature was 10℃ and light intensity was 150μmol/(m2·s), sodium alginate reached the maximum(2.867mg/g),when temperature was 20℃ and light intensity was 200umol/(m2·s), sodium alginate reached the minimum(2.438 mg/g).Carbohydrates content of Macrocystis pyrifera young sporophytes was significantly influenced by temperature, light intensity,but they was never influenced the interaction between the two factors.When light intensity was constant.as the temperature rose, carbohydrates content increased and then decreased.When temperature was constant, as light intensity rose, carbohydrates content increased and then decreased. Therefore, when temperature was 15℃ and light intensity was 150μmo/(m2·s).carbohydrates reached the maximum(34.56%), when temperature was 20℃ and light intensity was 50μmol/(m2·s), sodium alginate reached the minimum(24.41%).2.light intensity and salinity on Macrocystis pyrifera young sporophyte growth and biochemical compositionThe SGR of Macrocystis pyrifera young sporophytes was influenced by light intensity, temperature,but it was not influenced by the interaction between the two factors.When light intensity was constant, as salinity rose,SGR dropped after the first rise.When salinity was constant,as light intensity rose,SGR first increased and then decreased.Therefore,when light intensity was 100μmol/(m2·s) and salinity was 30, SGR reached maximum (12.098), when light intensity was 50μmol/(m2·s) and salinity was 20 salinity, SGR reached the minimum (2.297).The protein content of Macrocystis pyrifera young sporophytes was influenced by light intensity, temperature, and the interaction between the two factors.When light intensity was constant.as salinity rose,protein content was basically gradually increased.When salinity was constant, as light intensity rose.the protein content gradually increased. Therefore, the light intensity was 200μmol/(m2·s) and the salinity was 35, protein content reached the maximum (14.763mg/g), when salinity was 20 and light intensity was 50μmol/(m2·s), protein content reached the minimum (4.003mg/g).The chlorophyll a of Macrocystis pyrifera young sporophytes was influenced by light intensity.temperature,and the interaction between the two factors.when light intensity was constant, as salinity rose, chlorophyll a content was gradually increased. When salinity was constant, as light intensity rose, chlorophyll was gradually reduced. Therefore, when light intensity was 50μmol/(m2·s) and salinity was 35. chlorophyll a reached maximum (0.592mg/g), when light intensity was 200lmol/(m2·s) and salinity was 20. chlorophyll a reached the minimum (0.265mg/g). The chlorophyll c of Macrocystis pyhfera young sporophytes was influenced by light intensity, temperature, but it was not influenced by the interaction between the two factors.When the light intensity was constant, as salinity rose, chlorophyll c content was basically dropped after the first rise, but the light group 50lmol/(m2·s) had not such trend. When salinity was constant,as light intensity rose, the chlorophyll c content in between groups was not significantly different light intensity. Therefore, when light intensity was 50μmol/(m2·s)and was salinity 35. chlorophyll c content reached the maximum(2.463mg/g).when light intensity was 200μmol/(m2·s) and salinity was 20, chlorophyll c content reached the minimum (1.249mg/g).The carbon content of Macrocystis pyrifera young sporophytes was influenced by light intensity.but it was never influenced by salinity, and the interaction between the two factors.When light intensity was constant, as salinity rose, carbon content gradually increased. When salinity was constant, as light intensity rose, the carbon content decreased.When light intensity was 50μmol/(m2·s) and salinity was 35, the carbon content reached maximum (33.8%), when salinity was 20 and light intensity was 200μmol/(m2·s), the carbon content reached the minimum(26.5%). The nitrogen content of Macrocystis pyrifera young sporophytes was influenced by salinity,but it was never influenced by light intensity, and the interaction between the two factors.When light intensity was constant, as salinity rose, nitrogen content increased. When salinity was constant, as light intensity rose, nitrogen content increased and then decreased. Therefore, When light intensity was 100μmol/(m2·s) and salinity 35, nitrogen content reached maximum (5.33%), when salinity was 20 and light intensity was 50μmol/(m2·s), nitrogen content reached the minimum (4.24%).The sodium alginate content of Macrocystis pyrifera young sporophytes was influenced by light intensity,but it was never influenced by salinity, and the interaction between the two factors. When light intensity was constant, as salinity rose, sodium alginate content increased and then decreased, but the difference was not significant. When salinity was constant, as light intensity rose,sodium alginate content increased and then decreased. Therefore, when light intensity was 100μmol/(m2·s) and salinity was 30, sodium alginate reached maximum (2.678mg/g), when salinity was 20 and light intensity was 50μmol/(m2·s), sodium alginate reached the minimum (2.330 mg/g).The carbohydrates content of Macrocystis pyrifera young sporophytes was influenced by light intensity.salinity.but it was never influenced by the interaction between the two factors. When light intensity was constant, as salinity rose, carbohydrates content increased. When salinity was constant, as light intensity rose, carbohydrates content increased and then decreased. Therefore, when light intensity was 100umol/(m2·s) and salinity was 35, carbohydrates content reached maximum (38.15%), when salinity was 20 and light intensity was 200μmol/(m2·s), sodium alginate reached the minimum (30.10%).3.Tempreture and salinity on Macrocystis pyrifera young sporophyte growth and biochemical composition.When salinity was 20 and temperature 20℃. the algae die, blades disintegrated.The SGR of Macrocystis pyrifera young sporophytes was influenced by salinity, temperature, and the interaction between the two factors. When salinity was constant, as temperature rose, SGR increased and then decreased. When temperature was constant, as salinity rose,SGR increased and then decreased. Therefore, when salinity was 30 and temperature was 15℃, SGR reached the maximum (13.098), when salinity was 20 and temperature was 5℃, SGR reached the minimum (2.594).The protein content of Macrocystis pyrifera young sporophytes was influenced by salinity, temperature, and the interaction between the two factors. When salinity was constant, as the temperature rose, the protein content increased and then decreased. When the temperature was constant, as salinity rose, the protein content was substantially increased gradually. Therefore, when temperature was 10℃ and salinity was 35, protein content reached the maximum (17.178mg/g),when temperature was 5℃ and salinity was 20, protein content reached the minimum (1.024mg/g).The chlorophyll a content of of Macrocystis pyrifera young sporophytes was influenced by salinity, temperature, and the interaction between the two factors. When salinity was constant, as temperature rose, a change in chlorophyll a content had no regular trend, but when temperature was 20℃ chlorophyll a content in every salinity groups reached the maximum.When temperature was constant, as salinity rose, chlorophyll a content had basically gradual rise. Therefore, when salinity was 35 and temperature was 20℃, chlorophyll a content reached the maximum (1.226mg/g), when salinity was 20 and temperature was 5℃, chlorophyll a content reached the minimum (0.325mg/g). The chlorophyll c content of of Macrocystis pyrifera young sporophytes was influenced by salinity, temperature, and the interaction between the two factors. When salinity was constant, as temperature rose, a change in chlorophyll c content had no regular trend,but when temperature was 20℃ chlorophyll c content in every salinity groups reached the maximum.When temperature was constant.as salinity rose, chlorophyll c content c was substantially increased gradually. Therefore, when salinity was 35 and temperature was 20℃. chlorophyll c content reached the maximum (5.939mg/g). when salinity was 20 and temperature was 5℃. chlorophyll c content reached the minimum(1.584mg/g).The carbon content of Macrocystis pyrifera young sporophytes was influenced by temperature but it was not influenced by salinity or the interaction between the two factors.When salinity was constant, as temperature rose, the carbon decreased.When salinity was constant.When temperature was constant, as salinity rose, the carbon content increased and then decreased, but the difference was not significant. Therefore, when temperature was 25 and salinity was 5℃. carbon content reached the maximum (32.3%), when salinity was 35 and temperature was 20℃. carbon content reached the minimum (28.2%). Nitrogen content of of Macrocystis pyrifera young sporophytes was influenced by temperature but it was not influenced by salinity or the interaction between the two factors.When salinity was constant, as temperature rose, the nitrogen content did not differ significantly. When temperature was constant, as salinity rose,nitrogen content decreased. Therefore, when salinity was 20 and temperature was 15℃, nitrogen content reached the maximum (4.22%), when salinity was 35 and temperature was 5℃, nitrogen content reached the minimum (3.21%).The sodium alginate content of Macrocystis pyrifera young sporophytes was not influenced by salinity, temperature, or the interaction between the two factors.When temperature was constant, as salinity rose, sodium alginate content increased and then decreased. When salinity was constant, as temperature rose.sodium alginate content increased and then decreased.Therefore when salinity was 30 and temperature was 15℃, sodium alginate content reached the maximum (2.653mg/g), when salinity was 20 temperature was 5℃, sodium alginate content reached the minimum (2.435mg/g).The carbohydrates content of Macrocystis pyrifera young sporophytes was influenced by salinity,temperature,but it was not influenced by the interaction between the two factors.When temperature was constant, as salinity rose, carbohydrates content increased and then decreased. When salinity was constant.as temperature rose, carbohydrates content increased and then decreased.Therefore when salinity was 30 and temperature was 15℃, carbohydrates content reached the maximum (31.88%), when salinity was 2 temperature was 5℃, carbohydrates content reached the minimum (31.28%). |
PDF Full Text Request