| Crop straw biomass which can through processing converted into energy, cause its rich resources, low price, is high-profile. Use crops straw biomass as a raw material for hydrogen production by photosynthetic organisms is an important research direction of biomass energy utilization, but most of the microorganisms can not directly use digest cellulose and hemicellulose and other macromolecular compounds, therefore need a pretreatment of biomass materials before biomass as the light also hydrogen fermentation substrates.This paper mainly adopts the combination pretreatment of the metal ions and microorganisms of photosynthetic production of hydrogen using corn straw, metal ions pretreatment has simple operation and low cost, biological methods pretreatment has environmental pollution-free, which belongs to the environmentally friendly biomass pretreatment method, adopt the method of joint of producing hydrogen with straw pretreatment technology experiments, using the variation in content of cellulose, hemicellulose and lignin and the reducing sugar yield to determine its pretreatment ability, thus class for crops straw biomass clean harmless pretreatment provides new way. The experimental results show that:1)After using ferric chloride treat on 40 mesh and 80 mesh corn straw,through processing cellulose, hemicellulose, and lignin content changes, filter out 40 mesh processing optimal processing group for Solid-liquid ratio 1/20 g/m L, concentration 0.5 mol/L of ferric chloride solution, in 100-108 ℃ reaction 30 min, after that, corn straw cellulose of content more not processing of material increased has 52.78%, hemicellulose reduced 12.38%, lignin increased 37.8%, 80 mesh processing optimal group for Solid-liquid ratio 1/20 g/m L, concentration 0.4 mol/L of ferric chloride solution, in 100-108 ℃ reaction 30 min, corn straw cellulose content increased 56.08%, hemicellulose reduced 13.06%, lignin increased 37.8%, By scanning electron microscopy(sem) analysis, surface structure have obvious damage, and 80 mesh straw surface structure damage to slightly obviously and 40 mesh of straw.2)By white-rot fungus of 40 mesh and 80 mesh and after dealing with the ferric chloride of 40 and 80 object of corn stover processing after the change of reducing sugar content in the matrix are compared, found that:a. White-rot fungus treat on 40 mesh corn straw, reducing sugar content in the matrix down first, then rising and falling, about 24 days in vaccination white-rot fungus processing, reducing sugar content reached a peak of about 0.98 mg/ml, and then tends to be stable, the appropriate vaccination photosynthetic hydrogen producing bacteria for hydrogen production; After ferric chloride treatment of 40 mesh maize straw in white-rot fungus inoculation treatment, the reducing sugar content increased to a peak first, then decreases, finally stabilized about 12 days, reducing sugar content in the peak of about 1.92 mg/ml, the suitable inoculation photosynthetic bacteria for hydrogen production, hydrogen production by white-rot fungus dealing directly with 40 mesh corn straw suitable inoculation photosynthetic hydrogen producing bacteria for 12 days in advance, and the reducing sugar content in the high of 0.94 mg/ml, more suitable for photosynthetic bacteria to produce hydrogen.b.80 mesh corn straw after vaccination white-rot fungus, reducing sugar content in falling up then slow down again, first by white-rot fungus in about 24 days of the reducing sugar content in small peak at about 0.99 mg/m L, then tends to be stable, the appropriate vaccination photosynthetic hydrogen producing bacteria for hydrogen production; And 80 mesh corn straw treated with ferric chloride, after vaccination white-rot fungus, reducing sugar content was increased first and then decreases, finally tends to be stable, in dealing with about 12 days to reach a maximum of 2.52 mg/m L, the suitable inoculation photosynthetic bacteria for hydrogen production, hydrogen production by white-rot fungus dealing directly with 80 mesh corn straw suitable inoculation photosynthetic hydrogen producing bacteria for 12 days in advance, and the reducing sugar content in the high of 1.53 mg/m L, more suitable for photosynthetic bacteria to produce hydrogen.c. 40 and 80 mesh corn straw direct inoculation after white-rot fungus, have the same change of reducing sugar content in the matrix, are less in the slow increase and decrease, and finally tends to be stable, in the direct processing of white-rot fungus 24 days the most suitable for photosynthetic bacterium producing hydrogen, 80 mesh matrix of the reducing sugar content in corn stalk should be slightly higher than 40 purposes; White-rot fungus treatment by ferric chloride after 40 and 80 object of corn straw, matrix content of reducing sugar in basically the same change trend, all is first increase and decrease, and finally tends to be slow and steady, the inoculation of white-rot fungus 12 days the most suitable for photosynthetic bacterium producing hydrogen, 80 mesh matrix of the reducing sugar content in corn stalk to little more than 40 mesh.3) After processing of cellulose, hemicellulose, and lignin content change and the change of reducing sugar content, select the most optimal pretreatment method is: first the corn straw crushing to 80 mesh, take the 1/20 g/m L of solid-liquid ratio, concentration of 0.4 mol/L ferric chloride solution, in 100-108 ℃ for 30 min, after washing to neutral, reinoculation white-rot fungus processing about 12 days, for photosynthesis to produce hydrogen. |
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